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1.05235

Sigma-Aldrich

Cresyl violet (acetate)

for microscopy Certistain®

Synonym(s):

Cresyl violet (acetate)

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About This Item

Empirical Formula (Hill Notation):
C18H15N3O3
CAS Number:
Molecular Weight:
321.33
MDL number:
UNSPSC Code:
41116130
EC Index Number:
234-043-3

Quality Level

form

solid

composition

Dye, ≥70% spectrophotometric assay

pH

3 (20 °C, 10 g/L in H2O)

mp

140-143 °C

solubility

70 g/L

bulk density

460 kg/m3

εmax

980-1400 at 596-599 nm in 50% ethanol at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C16H12N3O.C2H4O2/c17-9-5-6-13-14(7-9)20-15-8-12(18)10-3-1-2-4-11(10)16(15)19-13;1-2(3)4/h1-8H,17-18H2;1H3,(H,3,4)/q+1;/p-1

InChI key

XKOCOMKJPWEOHX-UHFFFAOYSA-M

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General description

The dry dye Cresyl violet (acetate) for microscopy Certistain®, is used for the preparation of a staining solution that can be used for the staining of nuclei and Nissl bodies. Cresyl violet (acetate) belongs to the group of oxazine dyes. As a CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 70%
Identity
- acetate:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (ethanol 50%): 596 - 599 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; ethanol 50%): 980 - 1400
TLC-Test: passes test
Loss on drying (50 °C / <1 hPa): ≤ 25%
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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