07-1820
Anti-EEA1 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
early endosome antigen 1, early endosome-associated protein, early endosome antigen 1, 162kD, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
packaging
antibody small pack of 25 μL
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... EEA1(8411)
Related Categories
General description
Early endosome antigen 1 (EEA1) is a 170 kDa coiled-coil protein belonging to a group of early endosomal Rab5 effector proteins. EEA1 has a putative role in the tethering/docking of endocytic vesicles prior to their fusion with early endosomes. This protein is characterized as containing a zinc finger-like domain and coiled-coil regions. The zinc finger-like domain, also known as the FYVE finger, is a motif rich in cystine, is a necessary component of EEA1’s ability for endosomal targeting, and is specifically known for its dual interaction with Rab5 GTPase and phosphotidylinositol 3-phosphate (Ptdlns-3-P).
Specificity
Equine and Canine (99% sequence homology). Rat (98% sequence homology). Bovine (97% sequence homology).
This antibody recognizes EEA1.
Immunogen
Epitope: C-terminus
GST-tagged recombinant protein corresponding to the C-terminus of human EEA1.
Application
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
This Anti-EEA1 Antibody is validated for use in WB, IC for the detection of EEA1.
Western Blot (SNAP ID) Analysis: 1:250 dilution from a previous lot detected EEA1 on 10 µg of C2C12 cell lysate.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected EEA1 in HeLa, A431, and C2C12 cells.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected EEA1 in HeLa, A431, and C2C12 cells.
Quality
Evaluated by Western Blot in BALB 3T3/A31 cell lysate.
Western Blot Analysis: A 1:1000 dilution of this antibody detected EEA1 on 10 µg of BALB 3T3/A31 cell lysate.
Western Blot Analysis: A 1:1000 dilution of this antibody detected EEA1 on 10 µg of BALB 3T3/A31 cell lysate.
Target description
~ 170 kDa
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
BALB 3T3/A31 cell lysate
BALB 3T3/A31 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
recommended
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Matylda B Mielcarska et al.
Frontiers in cellular neuroscience, 14, 544612-544612 (2020-12-08)
TLR3 provides immediate type I IFN response following entry of stimulatory PAMPs into the CNS, as it is in HSV infection. The receptor plays a vital role in astrocytes, contributing to rapid infection sensing and suppression of viral replication, precluding
Songlin Li et al.
Frontiers in immunology, 11, 49-49 (2020-02-23)
Impaired synaptic integrity and function due to accumulation of amyloid β-protein (Aβ42) oligomers is thought to be a major contributor to cognitive decline in Alzheimer's disease (AD). However, the exact role of Aβ42 oligomers in synaptotoxicity and the ability of
Neurotrophin receptor p75 mediates the uptake of the amyloid beta (A?) peptide, guiding it to lysosomes for degradation in basal forebrain cholinergic neurons.
Ovsepian, Saak V, et al.
Brain Structure &Amp; Function (2013)
Liang Shi et al.
The international journal of biochemistry & cell biology, 90, 93-102 (2017-07-25)
The neuron-enriched, endosomal protein Calcyon (Caly) regulates endocytosis and vesicle sorting, and is important for synaptic plasticity and brain development. In the current investigation of Caly interacting proteins in brain, the microtubule retrograde motor subunit, cytoplasmic dynein 1 heavy chain
Sébastien A Gauthier et al.
Acta neuropathologica communications, 5(1), 65-65 (2017-08-31)
A dysfunctional endosomal pathway and abnormally enlarged early endosomes in neurons are an early characteristic of Down syndrome (DS) and Alzheimer's disease (AD). We have hypothesized that endosomal material can be released by endosomal multivesicular bodies (MVBs) into the extracellular
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service