05-1324
Anti-phospho-Histone H1 Antibody, clone 12D11
culture supernatant, clone 12D11, from mouse
Synonym(s):
H1 histone family, member 5, Histone H1a, histone 1, H1b, histone cluster 1, H1b
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
12D11, monoclonal
species reactivity
human, mouse, bovine, vertebrates
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgMκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (not specified)
Gene Information
bovine ... H1-5(527304)
human ... H1-5(3009)
General description
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures.
Specificity
Broad species cross-reactivity is expected, based on sequence homology.
Recognizes phosphorylated Histone H1, Mr 32 kDa.
Immunogen
The immunogen was KLH-conjugated bovine nuclear extract.
Application
Immunohistochemistry: This antibody has been reported by an independent laboratory to detect CDK (cyclin dependent kinase) phosphorylated Histone H1 in benign tissue sections.1
Immunocytochemistry:
This antibody has been demonstrated by an outside laboratory to show positive immunostaining for phosphorylated histone H1 in HUSK (human fetal primary myoblasts) cells fixed with 3.7% paraformaldehyde followed by permeabilization with 0.2% Triton X-100.2
Immunoprecipitation:
This antibody has been demonstrated by an outside laboratory to immunoprecipitate phosphorylated histone H1 using solid phase beads bound to anti-mouse IgM.
Immunocytochemistry:
This antibody has been demonstrated by an outside laboratory to show positive immunostaining for phosphorylated histone H1 in HUSK (human fetal primary myoblasts) cells fixed with 3.7% paraformaldehyde followed by permeabilization with 0.2% Triton X-100.2
Immunoprecipitation:
This antibody has been demonstrated by an outside laboratory to immunoprecipitate phosphorylated histone H1 using solid phase beads bound to anti-mouse IgM.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-phospho-Histone H1 Antibody, clone 12D11 (Mouse Monoclonal Antibody) validated in IHC, ICC, IP, WB to detect phospho-Histone H1 also known as Histone H1a, histone 1 H1b.
Quality
Western Blot Analysis:
1:1000 dilution of this antibody detected Histone H1 in colcemid-treated HeLa lysate.
1:1000 dilution of this antibody detected Histone H1 in colcemid-treated HeLa lysate.
Target description
~32 kDa
Physical form
Supplied as 200 μL of culture supernatant with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HeLa cell lysate, treated with colcemid.
HeLa cell lysate, treated with colcemid.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Growth factors that repress myoblast differentiation sustain phosphorylation of a specific site on histone H1.
Cole, F, et al.
The Journal of Biological Chemistry, 268, 1580-1585 (1993)
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