810601P

16:0-5 Doxyl PC

Avanti Research^™ - A Croda Brand 810601P, powder

• Synonym(s): 1-palmitoyl-2-stearoyl-(5-doxyl)-sn-glycero-3-phosphocholine
• Empirical Formula (Hill Notation): C₄₆H₉₀N₂O₁₀P
• CAS Number: 188004-24-2
• Molecular Weight: 862.19
• UNSPSC Code: 41141825
• NACRES: NA.25

Properties

• Assay: >99% (TLC)
• form: powder
• packaging: pkg of 1 × 1 mg (810601P-1mg)
• manufacturer/tradename: Avanti Research^™ - A Croda Brand 810601P
• lipid type: ESR probes; phospholipids
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Avanti′s nitroxide spin product listing is a group of compounds designed to act as membrane probes. A variety of positions down the hydrophobic chain are labeled with the nitroxide functional groups to allow probing the membrane at various depths. These compounds have been synthesized from 1-palmitoyl-2-hydroxy-sn-glycerol-3-phosphocholine with the product being purified by column chromatography. Various n-doxyl phosphocholines have been recently used as biophysical tools to elucidate membrane trafficking with phosphatidylinositol transfer proteins and as fluorescent quenchers in lipid bilayer structural studies.

Phosphatidylcholine (PC), a strong bilayer-forming lipid is the most common phospholipid in mammalian membranes. The excretory and secretory products of helminths has a small hapten-like portion called phosphorylcholine (PC). The 5^th carbon of the sn-2 stearic acid chain of 1-palmitoyl-2-stearoyl-(5-doxyl)-sn-glycero-3-phosphocholine analog has a Doxyl PC, a spin probe attached to it covalently.

Application

16:0-5 Doxyl PC may be used:

• as a component in virus-like large unilamellar vesicles (VL LUVs) to quench 4-chloro-7-nitrobenz-2-oxa-1,3-diazole (NBD) fluorescence emission
• in the preparation of multi-lamellar vesicles (MLVs) as a site-specific quencher to perform fluorescence quenching studies
• in the preparation of spin-labelled multi-lamellar vesicles (MLVs)

Biochem/physiol Actions

Phosphatidylcholine (PC) lowers the levels of cholesterol and triglycerides.

Packaging

5 mL Clear Glass Sealed Ampule (810601P-1mg)

Preparation Note

Product use: To prevent aggregation, prepare water-based solutions of 2 mM stock solutions of n-DOXYL PCs and store in plastic. Dilute stock solutions to 0.03- 0.1 mM solutions for EPR studies. For liposome preparations in fluorescent quenching measurements, dissolve the doxyl lipid in 150 μl absolute ethanol for a concentration of 40.3 mM , Additional supplemental information.

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids