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  • CSF-1 controls cerebellar microglia and is required for motor function and social interaction.

CSF-1 controls cerebellar microglia and is required for motor function and social interaction.

The Journal of experimental medicine (2019-07-28)
Veronika Kana, Fiona A Desland, Maria Casanova-Acebes, Pinar Ayata, Ana Badimon, Elisa Nabel, Kazuhiko Yamamuro, Marjolein Sneeboer, I-Li Tan, Meghan E Flanigan, Samuel A Rose, Christie Chang, Andrew Leader, Hortense Le Bourhis, Eric S Sweet, Navpreet Tung, Aleksandra Wroblewska, Yonit Lavin, Peter See, Alessia Baccarini, Florent Ginhoux, Violeta Chitu, E Richard Stanley, Scott J Russo, Zhenyu Yue, Brian D Brown, Alexandra L Joyner, Lotje D De Witte, Hirofumi Morishita, Anne Schaefer, Miriam Merad
ABSTRACT

Microglia, the brain resident macrophages, critically shape forebrain neuronal circuits. However, their precise function in the cerebellum is unknown. Here we show that human and mouse cerebellar microglia express a unique molecular program distinct from forebrain microglia. Cerebellar microglial identity was driven by the CSF-1R ligand CSF-1, independently of the alternate CSF-1R ligand, IL-34. Accordingly, CSF-1 depletion from Nestin+ cells led to severe depletion and transcriptional alterations of cerebellar microglia, while microglia in the forebrain remained intact. Strikingly, CSF-1 deficiency and alteration of cerebellar microglia were associated with reduced Purkinje cells, altered neuronal function, and defects in motor learning and social novelty interactions. These findings reveal a novel CSF-1-CSF-1R signaling-mediated mechanism that contributes to motor function and social behavior.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Collagenase from Clostridium histolyticum, suitable for release of physiologically active rat hepatocytes, Type IV, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid
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Ser-Phe-Leu-Leu-Arg-Asn-Pro-Asn-Asp-Lys-Tyr-Glu-Pro-Phe, ≥97% (HPLC)
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Biocytin, ≥98% (TLC)
Sigma-Aldrich
Human IL34 / Interleukin-34 ELISA Kit
Sigma-Aldrich
Corn oil, delivery vehicle for fat-soluble compounds
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Tamoxifen, ≥99%