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Methyl groups as NMR probes for biomolecular interactions.

Methyl groups as NMR probes for biomolecular interactions.

Current opinion in structural biology (2015-09-26)

Silke Wiesner, Remco Sprangers

ABSTRACT

Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that methodological advances and biological insights go hand in hand. We envision that future methyl TROSY applications will continue to provide unique information regarding intermolecular interactions, even for very large eukaryotic protein complexes that are often highly asymmetric.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

DLAM-M^εV^proS-¹³CH₃ Methyl Labeling Kit

Sigma-Aldrich

DLAM-LV^proS-¹³CHD₂ Methyl Labeling Kit

Sigma-Aldrich

HLAM-A^βI^δ1M^εLV^proST^γ-¹³CH₃ Methyl Labeling Kit

Sigma-Aldrich

DLAM-A^βV^proS-¹³CH₃ Methyl Labeling Kit

Sigma-Aldrich

DLAM-I^δ1T^γ-¹³CH₃ Methyl Labeling Kit

Sigma-Aldrich

TLAM-I^δ1M^εT^γ-¹³CH₃ Methyl Labeling Kit

Sigma-Aldrich

SLAM-A^β-¹³CH₃ Methyl Labeling Kit

Sigma-Aldrich

PLAM-A^βI^δ1LV^ProST^γ-¹³CH₃ Methyl Labeling Kit

Sigma-Aldrich

TLAM-^δI1LV^proS-¹³CH₃ Methyl Labeling Kit

Sigma-Aldrich

TLAM-I^δ1LVproR-U-¹³C Methyl Labeling Kit

Sigma-Aldrich

SLAM-V^proR-¹³CH₃ Methyl Labeling Kit

Sigma-Aldrich

SLAM-V^proS-¹³CH₃ Methyl Labeling Kit

Sigma-Aldrich

SLAM-A^β-¹³CHD₂ Methyl Labeling Kit

Sigma-Aldrich

DLAM-LV^proS-¹³CH₃ Methyl Labeling Kit

Sigma-Aldrich

DLAM-LV^proR-¹³C₄ Methyl Labeling Kit

Sigma-Aldrich

SLAM-V^proS-¹³CHD₂ Methyl Labeling Kit

Sigma-Aldrich

QLAM-I^δ1T^γLV^proS-¹³CH₃ Methyl Labeling Kit

Sigma-Aldrich

DLAM-LV^proR-¹³CH₃ Methyl Labeling Kit

Sigma-Aldrich

DLAM-I^δ1V^proS-¹³CH₃ Methyl Labeling Kit

Sigma-Aldrich

SLAM-I^γ2-¹³CH₃ Methyl Labeling Kit