V5265

Crystal Violet Solution

1%, aqueous solution

• Empirical Formula (Hill Notation): C₂₅H₃₀ClN₃
• CAS Number: 548-62-9
• Molecular Weight: 407.98
• Beilstein: 3580948
• MDL number: MFCD00011750
• UNSPSC Code: 12171500
• PubChem Substance ID: 329829057
• NACRES: NA.47

Properties

• form: aqueous solution
• concentration: 1%
• technique(s): microbe id | staining: suitable
• color: deep violet, purple
• Amax: 0.36-0.44 at 589-594 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C
• InChI: 1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
• InChI key: ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Description

General description

Crystal violet, also known as gentian violet or methyl violet, is the N-hexamethylated derivative of pararosaniline. It is a basic, cationic, acidotropic protein dye used in histological and bacteriological staining. It is well-known for its antibacterial and antifungal properties.

It stains the fatty portions of sebaceous sweat a deep purple color. Crystal violet can also be used to enhance bloody fingerprints. This dye is harmful if inhaled, swallowed or absorbed through skin, contact may cause cancer, severe eye irritation in human beings.

Application

• Crystal violet is commonly used in Gram staining for the classification of bacteria.
• It has also been used to detect bacterial adherence to biomedical polymers and to stain DNA in mammalian tissues in Giemsa staining.
• It has been successfully used to develop a counterion-staining method to detect DNA in agarose gel electrophoresis.
• Its antimicrobial properties have facilitated its use in the treatment of oral candidiasis, skin infections, and methicillin-resistant Staphylococcus aureus.
• It has been used as a stain in cell proliferation assays, migration assays, and Boyden chamber assay.

Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining.

Biochem/physiol Actions

The antimicrobial effects of crystal violet can be attributed to free radical generation and formation of unionized bacterial complexes with the dye. It has also been reported to inhibit protein synthesis and bacterial cell wall formation. It is especially effective against Gram-positive bacteria.

Principle

Crystal violet is the primary stain in Gram staining. It infiltrates the peptidoglycan-rich cell wall of Gram-positive bacteria, and forms entrapped complexes with Gram′s iodine that retain the purple color post-decolorization. Thus, Gram-positive bacteria can be differentiated from Gram-negative bacteria whose peptidoglycan layer is considerably thinner.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Warning
• Hazard Statements: H319,H351,H412
• Precautionary Statements: P202 - P264 - P273 - P280 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable