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Merck

T9822

Sigma-Aldrich

Anti-Tubulin, Polyglutamylated antibody, Mouse monoclonal

clone B3, purified from hybridoma cell culture

Synonym(s):

Anti-K-ALPHA-1

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

Pricing and availability is not currently available.

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

B3, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

mouse, sea urchin (Lytechinus pictus and three others.), bovine, monkey, rabbit, human, rat, Oxyrrhis marina (dinoflagellate), chicken

technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using cytosolic fraction of rat brain extract

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T7816T6074T6199
species reactivity

mouse, sea urchin (Lytechinus pictus and three others.), bovine, monkey, rabbit, human, rat, Oxyrrhis marina (dinoflagellate), chicken

species reactivity

Xenopus, chicken, human, hamster, canine, mouse, bovine, rat

species reactivity

mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, kangaroo rat

species reactivity

yeast, mouse, amphibian, human, rat, chicken, fungi, bovine

Gene Information

human ... TUBA4A(7277), TUBB(203068)
mouse ... Tuba1a(22142), Tubb2b(73710)
rat ... Tuba1a(64158)

Gene Information

human ... TUBB(203068)
mouse ... Tubb4(22153)

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

General description

Tubulin is a 100kD protein that is present in all cells as a heterodimer of two similar polypeptides α and β which assemble to form microtubules. Proper organization of microtubules is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-tubulin, polyglutamylated antibody can be used as primary antibody in colocalization experiments. This antibody can also be used for studies involving the inhibition of flagellar motility in permeabilized sperm models. Mouse anti-tubulin, polyglutamylated antibody reacts specifically with the epitope present in the C-terminal region of α and β tubulins (glutamylated motif at amino acids 445-457 of α-tubulin).
Tubulin is a cellular colchicine-binding protein.

Immunogen

purified sea urchin (Lytechinus pictus) sperm axonemal proteins.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-Tubulin, Polyglutamylated antibody produced in mouse has been used in:
  • immunoprecipitation
  • immunohistochemistry
  • immunofluorescence

Monoclonal anti-tubulin, polyglutamylated antibody can be used in immunoblotting, immunocytochemistry and ELISA. It can also be used as primary antibodies (diluted 1:1000) in western blotting.

Biochem/physiol Actions

Microtubule plays an important role in DNA segregation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Insight into tubulin regulation from a complex with colchicine and a stathmin-like domain
Ravelli RBG, et al.
Nature, 428(6979), 198-198 (2004)
Jenny Yamauchi et al.
Human molecular genetics, 22(21), 4306-4317 (2013-06-19)
Merosin-deficient congenital muscular dystrophy type 1A (MDC1A) is an autosomal recessive disorder caused by mutations in the laminin-α2 gene (OMIM: 607855). Currently, no treatment other than palliative care exists for this disease. In our previous work, genetic interventions in the
SDCCAG8 interacts with RAB effector proteins RABEP2 and ERC1 and is required for hedgehog signaling
Airik R, et al.
PLoS ONE, 11(5), e0156081-e0156081 (2016)
Olga V Plotnikova et al.
Methods in cell biology, 94, 137-160 (2009-01-01)
Cilia are microtubule-based structures that protrude from the cell surface and function as sensors for mechanical and chemical environmental cues that regulate cellular differentiation or division. In metazoans, ciliary signaling is important during organismal development and in the homeostasis controls
Karel Soucek et al.
The Prostate, 66(9), 954-965 (2006-03-17)
Multiple diverse posttranslational modifications of alpha-tubulin such as detyrosination, further cleavage of the penultimate glutamate residue (Delta2-tubulin), acetylation, and polyglutamylation increase the structural and functional diversity of microtubules. Herein, we characterized the molecular profile of alpha-tubulin posttranslational modifications in normal

Articles

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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