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T2145

Sigma-Aldrich

Tyrode′s Salts

Powder, without sodium bicarbonate, suitable for cell culture

Synonym(s):

Tyrode′s Buffer, Tyrode′s Solution, Tyrode′s buffer

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

Tyrode′s Salts, Without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

Related Categories

General description

Tyrode′s Salts play an important role in tissue culture. A salt solution′s basic function is to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

Application

Tyrode′s Salts has been used:
  • for seeding cardiomyocytes culture for optical based phenotyping and imaging studies
  • to incubate 3T3-L1 pre-adipocytes in calcium intracellular assays
  • as a medium for the isolation and culturing of myofiber extensor digitorum longus (EDL) muscle

Quantity

Formulated to contain 9.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.0 g/L sodium bicarbonate.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Theodora Pavlidou et al.
Stem cells international, 2019, 5980465-5980465 (2019-06-30)
The regeneration of the muscle tissue relies on the capacity of the satellite stem cell (SC) population to exit quiescence, divide asymmetrically, proliferate, and differentiate. In age-related muscle atrophy (sarcopenia) and several dystrophies, regeneration cannot compensate for the loss of
Yu-Fen Chang et al.
PloS one, 12(4), e0174181-e0174181 (2017-04-06)
Identification of drug induced electrical instability of the heart curtails development, and introduction, of potentially proarrhythmic drugs. This problem usually requires complimentary contact based approaches such as patch-clamp electrophysiology combined with field stimulation electrodes to observe and control the cell.
Amalia Pérez-Jiménez et al.
Phytomedicine : international journal of phytotherapy and phytopharmacology, 23(12), 1301-1311 (2016-10-22)
Metabolic syndrome is a set of pathologies among which stand out the obesity, which is related to the lipid droplet accumulation and changes to cellular morphology regulated by several molecules and transcription factors. Maslinic acid (MA) is a natural product
Felipe Mora-Bermúdez et al.
eLife, 3 (2014-07-06)
Mitotic spindle orientation is crucial for symmetric vs asymmetric cell division and depends on astral microtubules. Here, we show that distinct subpopulations of astral microtubules exist, which have differential functions in regulating spindle orientation and division symmetry. Specifically, in polarized
James F X Jones et al.
Experimental physiology, 88(3), 329-334 (2003-04-30)
The cranial venae cavae of the rat heart are composed of cardiac muscle. We tested whether the vagus nerve has an inotropic action on these blood vessels. Stimulation of right or left vagal fibres (n = 7 animals) produced a
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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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