S5193
SYBR® Green JumpStart™ Taq ReadyMix™
for quantitative PCR, without MgCl2 in buffer
Synonym(s):
Hot start PCR master mix, sybr green PCR master mix, sybr green qPCR
About This Item
Recommended Products
Quality Level
form
liquid
usage
sufficient for 100 reactions
sufficient for 20 reactions
sufficient for 400 reactions
feature
dNTPs included
hotstart
concentration
1.25 units/reaction (50 μL reaction volume)
technique(s)
qPCR: suitable
color
colorless
input
purified DNA
suitability
suitable for PCR
application(s)
agriculture
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1 of 4
This Item | 24157 | 25381 | 25358 |
---|---|---|---|
≤25-250 °C temperature (isothermal or programmed) | ≤25-230 °C temperature (isothermal or programmed) | ≤25-230 °C temperature (isothermal or programmed) | ≤25-230 °C temperature (isothermal or programmed) |
gas chromatography (GC): suitable | gas chromatography (GC): suitable | gas chromatography (GC): suitable | gas chromatography (GC): suitable |
capillary intermediate polar | capillary intermediate polar | capillary intermediate polar | capillary intermediate polar |
fused silica | fused silica | fused silica | fused silica |
environmental | environmental | environmental | environmental |
General description
Application
- During RNA extraction, reverse transcription and Quantitative PCR[1]
- During analysis of leptin and leptin receptor expression. (PCR and melting curve analyses were performed)[2]
- For tissue RNA Isolation. (PCR and melting curve analyses were performed)[3]
- in real-time PCR (RT-PCR) of reverse-transcribed cDNA template[4][5]
- For qPCR amplifications using SYBR
- For multiplex PCR
Features and Benefits
- Delivers the benefits of antibody-inactivated hot-start PCR with SYBR Green detection in a ReadyMix ideal for high throughput applications; only primers and template are required
- JumpStart™ Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yield
- SYBR® Green JumpStart™ Taq ReadyMix for SYBR based qPCR is formulated with MgCl2 or packaged with a separate vial for ease of optimization. ReadyMixes are compatible with tube- and plate-based instruments
- This master mix allows consistency from one reaction to the next
- Designed to minimize contamination
- Reduced primer dimers
- Reduced set-up time as compared to manual or wax HotStart® methods
- Allows for room temperature set-up and contains a fluorescent dye, which is ideal for qPCR applications
Packaging
20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
400RXN is packaged as 1 X 10 mL
500RXN is packaged as 1 X 12.5 mL
Other Notes
Principle
SYBR Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. The instrument settings for ROX reference dye are satisfactory for the measurement of the Reference Dye for Quantitative PCR. Specificity of Sigma′s SYBR based QPCR detection is greatly enhanced by the incorporation of a hot-start mediated taq polymerase, JumpStart Taq.
Legal Information
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Protocols
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
Related Content
SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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