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S5193

Sigma-Aldrich

SYBR® Green JumpStart Taq ReadyMix

for quantitative PCR, without MgCl2 in buffer

Synonym(s):

Hot start PCR master mix, sybr green PCR master mix, sybr green qPCR

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About This Item

MDL number:
UNSPSC Code:
41106300
NACRES:
NA.55

Pricing and availability is not currently available.

Quality Level

form

liquid

usage

sufficient for 100 reactions
sufficient for 20 reactions
sufficient for 400 reactions

feature

dNTPs included
hotstart

concentration

1.25 units/reaction (50 μL reaction volume)

technique(s)

qPCR: suitable

color

colorless

input

purified DNA

suitability

suitable for PCR

application(s)

agriculture

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This Item
241572538125358
parameter

≤25-250 °C temperature (isothermal or programmed)

parameter

≤25-230 °C temperature (isothermal or programmed)

parameter

≤25-230 °C temperature (isothermal or programmed)

parameter

≤25-230 °C temperature (isothermal or programmed)

technique(s)

gas chromatography (GC): suitable

technique(s)

gas chromatography (GC): suitable

technique(s)

gas chromatography (GC): suitable

technique(s)

gas chromatography (GC): suitable

column type

capillary intermediate polar

column type

capillary intermediate polar

column type

capillary intermediate polar

column type

capillary intermediate polar

material

fused silica

material

fused silica

material

fused silica

material

fused silica

application(s)

environmental
food and beverages
forensics and toxicology
industrial hygiene

application(s)

environmental
food and beverages
forensics and toxicology
industrial hygiene

application(s)

environmental
food and beverages
forensics and toxicology
industrial hygiene

application(s)

environmental
food and beverages
forensics and toxicology
industrial hygiene

General description

SYBR® Green JumpStart Taq ReadyMix combines the performance enhancements of JumpStart Taq antibody for hot start PCR with SYBR Green I and the convenience of an easy-to-use ReadyMix solution. This ready-to-use mixture of SYBR Green I, JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer is provided in a 2× concentrate for ease of use. Simply add 25 μL of the 2× mix to DNA template, primers and water. The JumpStart Taq antibody inactivates DNA polymerase at room temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active. JumpStart Taq DNA polymerase prevents non-specific amplification resulting in more accurate CT values.

Application

SYBR® Green JumpStart® Taq ReadyMix has been used:

  • During RNA extraction, reverse transcription and Quantitative PCR[1]
  • During analysis of leptin and leptin receptor expression. (PCR and melting curve analyses were performed)[2]
  • For tissue RNA Isolation. (PCR and melting curve analyses were performed)[3]
  • in real-time PCR (RT-PCR) of reverse-transcribed cDNA template[4][5]
  • For qPCR amplifications using SYBR
  • For multiplex PCR
SYBR® Green JumpStart Taq ReadyMix has been used:
  • to prepare the real-time PCR mixture[6]
  • in the quantitative analysis of gene expression by real time, quantitative polymerase chain reaction (qPCR)[7]
  • to determine the mRNA expression of nuclear factor erythroid 2 (NFE2)-related factor 2 (Nrf2) gene by qPCR[8]

Features and Benefits

  • Delivers the benefits of antibody-inactivated hot-start PCR with SYBR Green detection in a ReadyMix ideal for high throughput applications; only primers and template are required
  • JumpStart Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yield
  • SYBR® Green JumpStart Taq ReadyMix for SYBR based qPCR is formulated with MgCl2 or packaged with a separate vial for ease of optimization. ReadyMixes are compatible with tube- and plate-based instruments
  • This master mix allows consistency from one reaction to the next
  • Designed to minimize contamination
  • Reduced primer dimers
  • Reduced set-up time as compared to manual or wax HotStart® methods
  • Allows for room temperature set-up and contains a fluorescent dye, which is ideal for qPCR applications

Packaging

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
400RXN is packaged as 1 X 10 mL
500RXN is packaged as 1 X 12.5 mL

Other Notes

Magnesium chloride is provided separately for optimization.

Principle

SYBR Green JumpStart Taq ReadyMix is recommended for single product real-time amplification experiments and can also be used for PCR optimization prior to manufacture of fluorescent-labeled probes. Fluorescent labeled probes are not recommended for use with SYBR Green I dye.

SYBR Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. The instrument settings for ROX reference dye are satisfactory for the measurement of the Reference Dye for Quantitative PCR. Specificity of Sigma′s SYBR based QPCR detection is greatly enhanced by the incorporation of a hot-start mediated taq polymerase, JumpStart Taq.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Eppendorf is a registered trademark of Eppendorf AG
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
Julabo is a registered trademark of Julabo USA, Inc.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

Pictograms

Exclamation markEnvironment

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Nicholas Zoulias et al.
PloS one, 15(1), e0222480-e0222480 (2020-01-17)
Light is a crucial signal that regulates many aspects of plant physiology and growth including the development of stomata, the pores in the epidermal surface of the leaf. Light signals positively regulate stomatal development leading to changes in stomatal density
Kanta Chechi et al.
PloS one, 7(4), e32265-e32265 (2012-04-19)
Relative quantification is a commonly used method for assessing gene expression, however its accuracy and reliability is dependent upon the choice of an optimal endogenous control gene, and such choice cannot be made a priori. There is limited information available
Asad Zeidan et al.
The Journal of pharmacology and experimental therapeutics, 315(3), 1075-1084 (2005-09-08)
Various cardiovascular pathologies are associated with vascular smooth muscle cell (VSMC) hypertrophy and elevated plasma leptin levels. We used the rat portal vein (RPV) cultured for three days to investigate the effect of mechanical stretch on autocrine secretion of leptin
Brown fat like gene expression in the epicardial fat depot correlates with circulating HDL-cholesterol and triglycerides in patients with coronary artery disease
Chechi K, et al.
International Journal of Cardiology, 167(5), 2264-2270 (2013)
Nesrine S El Sayed et al.
Frontiers in pharmacology, 12, 666502-666502 (2021-08-10)
Sporadic Alzheimer's disease (AD) is the most common neurodegenerative disorder with cognitive dysfunction. Remarkably, alteration in the gut microbiome and resultant insulin resistance has been shown to be connected to metabolic syndrome, the crucial risk factor for AD, and also

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Protocols

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

Related Content

SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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