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Key Documents

S0615

Sigma-Aldrich

FBS Superior

Supplemented FBS, EU approved

Synonym(s):

FBS, FCS, Fetal Bovine Sera, Fetal Bovine Serum, Supplemented FCS

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

biological source

bovine fetus

Quality Level

400

sterility

sterile-filtered

product line

FBS Select

quality

EU approved

composition

hemoglobin, <50 mg/dL

origin

non-USA origin

impurities

≤25 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

General description

Fetal bovine serum (FBS), a traditional serum supplement, is used in animal cell culture media. It is obtained from the fetus of the cow.

Application

FBS Superior has been used:
  • as a component of differentiation medium, growth medium, induction medium, and Dulbecco′s modified Eagle medium (DMEM) for murine thermogenic adipocytes culture
  • as a component of the culture medium along with DMEM high glucose (HG) for cell viability check
  • as a component of minimum essential medium Eagle (MEM) to cultivate Caco-2 human colon adenocarcinoma cells

Biochem/physiol Actions

Fetal bovine serum(FBS) Superior is a South American source of FBS supplemented with various growth-promoting compounds to allow for a more consistent product. This supplementation can reduce or eliminate the need to test multiple batches on specific cell lines. FBS Superior can be used just like regular FBS, delivers nutrients, growth, and attachment factors, and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Fetal bovine serum (FBS) comprises growth factors (GFs) and other elements that are required for the expansion, maintenance, attachment, and proliferation of adipose-derived stromal cells (ASC) in vitro.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ali Samadi et al.
Advanced biomedical research, 10, 4-4 (2021-05-08)
Wounds have a bad prognostic nature and excessive discharges whose regular wound dressings are ineffective. Hydrogels are the best candidates for dressing such wounds due to their high water content and ability to exchange substances. Accordingly, the purpose of this
Carlo E A Jochems et al.
Alternatives to laboratory animals : ATLA, 30(2), 219-227 (2002-04-25)
Fetal bovine serum (FBS) is a common component of animal cell culture media. It is harvested from bovine fetuses taken from pregnant cows during slaughter. FBS is commonly harvested by means of a cardiac puncture without any form of anaesthesia.
Carla Dessels et al.
Frontiers in cell and developmental biology, 4, 115-115 (2016-11-02)
Adipose-derived stromal cells (ASCs) are being used extensively in clinical trials. These trials require that ASCs are prepared using good manufacturing practices (GMPs) and are safe for use in humans. The majority of clinical trials in which ASCs are expanded
Arjen J Jakobi et al.
Nature communications, 11(1), 440-440 (2020-01-25)
p62/SQSTM1 is an autophagy receptor and signaling adaptor with an N-terminal PB1 domain that forms the scaffold of phase-separated p62 bodies in the cell. The molecular determinants that govern PB1 domain filament formation in vitro remain to be determined and
Julia Huetter et al.
Cancer immunology research, 8(7), 895-911 (2020-04-22)
The immunoglobulin-like domain containing receptor 2 (ILDR2), a type I transmembrane protein belonging to the B7 family of immunomodulatory receptors, has been described to induce an immunosuppressive effect on T-cell responses. Besides its expression in several nonlymphoid tissue types, we
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