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R4408

Sigma-Aldrich

Chloramphenicol

Ready Made Solution, 100 mg/mL in ethanol: isopropanol (95:5), ≥98% (HPLC)

Synonym(s):

D-(-)-threo-2,2-Dichloro-N-[β-hydroxy-α−(hydroxymethyl)-β-(4-nitrophenyl)ethyl]acetamide, D-(-)-threo-2-Dichloroacetamido-1-(4-nitrophenyl)-1,3-propanediol, D-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-4-nitrophenethyl]acetamide

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About This Item

UNSPSC Code:
51102831
NACRES:
NA.85
Pricing and availability is not currently available.

Quality Level

200

Assay

≥98% (HPLC)

form

solution

concentration

100 mg/mL in ethanol: isopropanol (95:5)

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

Mode of action

protein synthesis | interferes

shipped in

dry ice

storage temp.

−20°C

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Application

Chloramphenicol is a synthetic antibiotic, isolated from strains of Streptomyces venezuelae. It is often used for bacterial selection in molecular biology applications at 10-20 μg/mL and as a selection agent for transformed cells containing chloramphenicol reistance genes.

Biochem/physiol Actions

Mode of Action: Chloramphenicol inhibits bacterial protein synthesis by blocking the peptidyl transferase step by binding to the 50S ribosomal subunit and preventing attachment of aminoacyl tRNA to the ribosome. It also inhibits mitochondrial and chloroplast protein synthesis and ribosomal formation of (p)ppGpp, de-pressing rRNA transcription.

Mode of Resistance: Use of chloramphenicol acetyltransferase will acetylate the product and inactivate it.

Antimicrobial Spectrum: This is a broad spectrum antibiotic against gram-positive and gram-negative bacteria, and is used mainly for ophthalmic and veterinary purposes.

Packaging

10ml

Preparation Note

This product is a ready made solution of 100 mg/mL in ethanol:isopropanol (95:5). It is shipped in dry ice and stored at -20°C.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.Storage class (TRGS 510): Flammable liquids")

Signal Word

Danger

Hazard Statements

H225,H318,H351,H361fd

Hazard Classifications

Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Repr. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

57.2 °F - closed cup

Flash Point(C)

14.0 °C - closed cup

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Certificates of Analysis (COA)

Lot/Batch Number
0000375881
0000367717
0000367717
0000297126
0000297126

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Stephanie Petrella et al.
Antimicrobial agents and chemotherapy, 52(10), 3725-3736 (2008-07-16)
Two clinical strains of Escherichia coli (2138) and Enterobacter cloacae (7506) isolated from the same patient in France and showing resistance to extended-spectrum cephalosporins and low susceptibility to imipenem were investigated. Both strains harbored the plasmid-contained bla(TEM-1) and bla(KPC-2) genes.
Young Chan Song et al.
Annals of dermatology, 23(3), 321-328 (2011-09-13)
Although acne is a common follicular inflammatory dermatosis, studies of the relationship between Malassezia yeasts and acne have rarely been conducted. We sought to identify Malassezia yeasts from acne patients and establish a relationship between specific types of species of
Volkmar Passoth et al.
Biotechnology for biofuels, 2(1), 16-16 (2009-08-22)
Drying is currently the most frequently used conservation method for cereal grain, which in temperate climates consumes a major part of process energy. Airtight storage of moist feed grain using the biocontrol yeast Pichia anomala as biopreservation agent can substantially
Yudong Liu et al.
Antimicrobial agents and chemotherapy, 53(2), 512-518 (2008-11-26)
Methicillin (meticillin)-resistant Staphylococcus aureus (MRSA) is a serious problem worldwide. To investigate the molecular epidemiology of MRSA isolates in China, a total of 702 MRSA isolates collected from 18 teaching hospitals in 14 cities between 2005 and 2006 were characterized
Johannes Zander et al.
Infection and immunity, 76(4), 1333-1339 (2007-12-28)
Thymidine-dependent small-colony variants (SCVs) of Staphylococcus aureus are frequently associated with persistent and recurrent infections in cystic fibrosis patients. The phenotypic appearance of S. aureus SCVs or normal-colony variants (NCVs) is postulated to be affected by the intracellular amount of

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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