P0071
Anti-PDCD4 antibody produced in rabbit
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-Neoplastic transformation inhibitor, Anti-Nuclear antigen H731, Anti-Programmed cell death 4, Anti-Protein 197/15a
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~51 kDa
species reactivity
human, mouse (predicted), rat (predicted)
concentration
~1 mg/mL
technique(s)
immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates
western blot: 2-4 μg/mL using K562 cell lysates
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PDCD4(272250)
mouse ... Pdcd4(18569)
rat ... SELP(6403)
General description
Programmed Cell Death 4 (PDCD4 ) is present in the nucleus. PDCD4 gene is mapped to human chromosome 10q25.2. PDCD4 protein consists of two basic domains at the N-terminus and C-terminus and two conserved α-helical MA-3 domains.
Specificity
Anti-PDCD4 specifically recognizes human, mouse, and rat PDCD4.
Application
Anti-PDCD4 antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.
Biochem/physiol Actions
Programmed Cell Death 4 (PDCD4) is a neoplastic transformation inhibitor protein that functions as a tumor suppressor in ovarian carcinoma. The levels of PDCD4 are depleted in progressed carcinomas of the lung, breast, colon, and prostate. MA-3 domain of PDCD4 aids protein-protein interactions in eukaryotic translation initiation factor 4GI(eIF4GI) and eIF4GII with the ATP-dependent RNA helicase eIF4A. PDCD4 suppresses translation initiation by specifically inhibiting the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. In response to mitogens, PDCD4 is rapidly phosphorylated on Ser67 by the protein kinase ribosomal protein S6 kinase 1 (S6K1), and subsequently degraded via the ubiquitin ligase skp, cullin, f-box containing complex beta-transducin repeat-containing protein ((SCF)βTRCP ). It also shows that the protein is specifically phosphorylated on Ser76 and Ser457 by p90 ribosomal S6 kinase (RSK).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Sachiko Matsuhashi et al.
International journal of molecular sciences, 20(9) (2019-05-12)
PDCD4 is a novel tumor suppressor to show multi-functions inhibiting cell growth, tumor invasion, metastasis, and inducing apoptosis. PDCD4 protein binds to the translation initiation factor eIF4A, some transcription factors, and many other factors and modulates the function of the
Brigitte Lankat-Buttgereit et al.
Biology of the cell, 101(6), 309-317 (2009-04-10)
Pdcd4 (programmed cell death 4) has been known as a tumour suppressor gene and potential target for anticancer therapies for several years. Initially, Pdcd4 was identified as a gene that is up-regulated during apoptosis, but its precise role still remains
Xialian Xu et al.
Kidney international, 82(11), 1167-1175 (2012-07-13)
Delayed ischemic preconditioning effectively protects kidneys from ischemia-reperfusion injury but the mechanism underlying renal protection remains poorly understood. Here we examined the in vivo role of microRNA miR-21 in the renal protection conferred by delayed ischemic preconditioning in mice. A
Sung-Chul Lim et al.
Oncology letters, 2(6), 1053-1057 (2012-10-11)
The aim of this study was to examine the role of Programmed cell death 4 (Pdcd4) in colorectal adenocarcinoma (CRA). Pdcd4 expression was observed in both the nucleus and cytoplasm in colorectal adenocarcinoma, whereas Pdcd4 was expressed in the nucleus
Jiali Long et al.
Gene, 680, 59-64 (2018-09-24)
In recent years, the incidence and mortality of colorectal cancer (CRC) have been on a global upward trend. There is an urgent need for effective tools to prevent and treat CRC and reduce morbidity and mortality of CRC patients. Recent
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