N2915

PYR-41

≥98% (HPLC), powder

• Synonym(s): 4[4-(5-Nitro-furan-2-ylmethylene)-3,5-dioxo-pyrazolidin-1-yl]-benzoic acid ethyl ester
• Empirical Formula (Hill Notation): C₁₇H₁₃N₃O₇
• CAS Number: 418805-02-4
• Molecular Weight: 371.30
• UNSPSC Code: 12352200
• PubChem Substance ID: 329818421
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥98% (HPLC)
• form: powder
• color: red to brown
• solubility: DMSO: 5 mg/mL, clear (warmed)
• storage temp.: −20°C
• SMILES string: CCOC(=O)c1ccc(cc1)N2NC(=O)\C(=C/c3ccc(o3)[N+]([O-])=O)C2=O
• InChI: 1S/C17H13N3O7/c1-2-26-17(23)10-3-5-11(6-4-10)19-16(22)13(15(21)18-19)9-12-7-8-14(27-12)20(24)25/h3-9H,2H2,1H3,(H,18,21)/b13-9+
• InChI key: ARGIPZKQJGFSGQ-UKTHLTGXSA-N

Description

Application

PYR-41 has been used as E1 inhibitor:

• to determine the cellular degradation pathways on adeno-associated viruses (AAV) transduction
• to assess its selectivity and potency by matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) E2/E3 assay
• to treat fully differentiated C2C12 myotubes in high glucose medium for determining its effect on protein ubiquitination and cell viability
• in the pretreatment of human brain microvascular endothelial cells (hBMECs) for the inhibition studies before infection with bacteria

Biochem/physiol Actions

PYR-41 inhibits E1 ubiquitin ligases like mouse double minute 2 homolog (MDM2), Itchy (ITCH) and HOIL-1L interacting protein (HOIP). It has anticancer potential.

PYR-41 is a cell permeable inhibitor of Ubiquitin activating enzyme (E1) with little or no activity against E3, E2, or caspase enzymatic activity.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H302,H317
• Precautionary Statements: P280 - P301 + P312 + P330 - P302 + P352
• Hazard Classifications: Acute Tox. 4 Oral - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable