No information is available on the use of this kit with formalin fixed paraffin embedded samples. Some concerns would be whether the sample could be effectively homogenized into an aqueous solution, and if the remaining formalin/formaldehyde would affect the enzymes involved in this assay.
MAK335
Succinate Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonym(s):
Succinate Quantification Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.
Recommended Products
usage
sufficient for 100 colorimetric or fluorometric tests
input
beverage(s)
food(s)
biological sample(s)
detection method
colorimetric
fluorometric
relevant disease(s)
cardiovascular diseases; immunological diseases
storage temp.
−20°C
General description
Succinate, or succinic acid, can be found in all plants and animal tissues. It is an intermediate in the citric acid cycle and plays an important role in intracellular energy generation. Succinate is widely used as a flavoring agent in the food, beverage, and pharmaceutical industries due to its low toxicity.
Application
The Succinate Assay Kit may be used for:
- Diabetes Research
- Food and Beverage Testing
- Mitochondrial Disorder Research
Features and Benefits
Efficient and Precise: Detect Succinate levels ranging from 10 to 400 μM (colorimetric) and 2 to 40 μM (fluorimetric) in a 30 minute room temperature reaction, providing both convenience and sensitivity.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Suitability
Suitable for succinate determination in food, beverage, agricultural products, and other biological samples.
Principle
The Succinate Assay Kit provides a simple, one step assay for measuring succinate. In this assay succinate is converted to pyruvate which reacts with specific reagents and dye to form a colored product. The color intensity at 570 nm or fluorescence at λex/em = 530/585 nm of the reaction product is directly proportional to succinate concentration in the sample. Linear detection range 10 μM to 400 μM for colorimetric assays and 2 to 40 μM for fluorimetric assays.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Storage Class Code
10 - Combustible liquids
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Sulagna Bhattacharya et al.
EBioMedicine, 68, 103418-103418 (2021-06-09)
We have previously described an evolutionarily selected Tibetan prolyl hydroxylase-2 (PHD2D4E;C127S) variant that degrades the hypoxia-inducible factor (HIFα) more efficiently and protects these highlanders from hypoxia-triggered elevation in haemoglobin concentration. High altitude is known to cause acute mountain sickness (AMS)
Nishith M Shrimali et al.
EBioMedicine, 73, 103672-103672 (2021-11-06)
Phospho-Akt1 (pAkt1) undergoes prolyl hydroxylation at Pro125 and Pro313 by the prolyl hydroxylase-2 (PHD2) in a reaction decarboxylating α-ketoglutarate (αKG). We investigated whether the αKG supplementation could inhibit Akt-mediated activation of platelets and monocytes, in vitro as well as in
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Hello. Regarding the Succinate Assay Kit can this kit be used on samples stored in formalin ie formalin fixed paraffin embedded blocks of human tissues?
1 answer-
Helpful?
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Bonjour, Je souhaiterais connaitre le niveau de sensibilité du test de dosage du succinate? avez-vous une expérience à partager de ce dosage dans des matrices biologiques diverses (homogénats de tissus ou de culture cellulaires). Je vous remercie.
1 answer-
This kit is suitable for use in food, beverage, agricultural products, and other biological samples. The linear detection range is 10 - 400 µM for colorimetric assays and 2 - 40 µM for fluorimetric assays. See below for general preparation instructions:
Cell Samples. Suspend about two million (2 x 106) harvested cells in 400 µL PBS on ice. Lysis can be achieved by homogenization (10-20 passes in a Dounce homeginer on ice) or by sonication (preferably performed in an ice-water bath). The degree of cell lysis can be checked under a microscope. Centrifuge homogenate at 14,000 g for 10 min. Transfer the clear supernatant into a clean tube. It is prudent to run a pilot test of the sample at different dilutions. Choose a dilution with the readings in the linear range of the standard curve for further assays. Most samples can be stored at -80°C if not assayed immediately.
Tissue Samples. Start with 20-100 mg tissue, add 200- 1000 µL ice-cold PBS. Lysis can be achieved by homogenization (10-20 passes in a Dounce homeginer on ice) or by sonication (preferably performed in an ice-water bath). The degree of tissue lysis can be checked under a microscope. Centrifuge homogenate at 14,000 g for 10 min. Transfer the clear supernatant into a clean tube. It is prudent to run a pilot test of the sample at different dilutions. Choose a dilution with the readings in the detection range of the standard curve for further assays. Most samples can be stored at -80°C if not assayed immediately.
Please see the kit below to review the complete datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/325/481/mak335bul.pdfHelpful?
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