M9808

Anti-Muscarinic Acetylcholine Receptor (M₁) antibody produced in rabbit

affinity isolated antibody, lyophilized powder

• Synonym(s): Acetylcholine Receptor antibody
• MDL number: MFCD02096983
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: mouse, rat
• technique(s): immunoblotting: 1:200 using rat brain membranes; immunohistochemistry: suitable
• UniProt accession no.: P08482
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CHRM1(1128) ; rat ... Chrm1(25229)

Description

General description

Muscarinic acetylcholine receptor 1 (M1) is a member of class A G protein-coupled receptors (GPCRs) family, which is encoded by the CHRM1 gene. Muscarinic receptors have seven transmembrane domains localized in the parasympathetic nerve sub-synaptic membrane. The CHRM1 gene is located on human chromosome location 11q12.3.

Muscarinic cholinergic receptor 1 (M1) is seen in the forebrain of rats.

Immunogen

GST fusion protein of a part of the i3 intracellular loop of human M₁ muscarinic acetylcholine receptor (mAChR) corresponding to amino acid residues 227-353.^{1, 2}

Application

Anti-Muscarinic acetylcholine receptor (M1) antibody produced in rabbit has been used in immunohistochemistry and Western blotting.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)

Biochem/physiol Actions

Muscarinic acetylcholine receptor 1 (M1) regulates normal prostate growth and reduced-level glandular secretion. Muscarinic receptors play a major role in tumor progression. Muscarinic acetylcholine receptors (mAChRs) regulates adenylyl cyclase and potassium channel activities. It also stimulates various mitogen-activated protein kinases. M1 may be useful for the discovery of therapeutics for Alzheimer′s disease.

Studies have shown that activation of muscarinic cholinergic receptor 1 (M1) in rats leads to changes in the endotoxin tolerance in the liver.

Physical form

Lyophilized from a solution of phosphate-buffered saline, pH 7.4, containing 1% bovine serum albumin, and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable