F6928

Monoclonal Anti-FKHR (FOXO1a) antibody produced in mouse

clone FKH 117, purified immunoglobulin, buffered aqueous solution

• MDL number: MFCD03791549
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: FKH 117, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 70-75 kDa
• species reactivity: human
• technique(s): immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.5-1 μg/mL using total extract of COS-7 cells transfected with FKHR expression plasmid
• isotype: IgG1
• UniProt accession no.: Q12778
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FOXO1(2308)

Description

General description

FKHR belongs to the superfamily of forkhead transcription factors (FOX). It is characterized by a common Forkhead (or winged helix) domain, a variant of the helix-turn-helix motif. FKHR contains three residues, Thr24, Ser256, and Ser319 that lie within consensus sequences for phosphorylation by protein kinase B (PKB).

Monoclonal Anti-FKHR (FOXO1a) (mouse IgG1 isotype) is derived from the FKH117 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to human FKHR, conjugated to KLH.

Immunogen

synthetic peptide corresponding to human FKHR (amino acids 636-651), conjugated to KLH.

Application

Monoclonal Anti-FKHR (FOXO1a) antibody is suitable for immunoblot assay to study the down-regulation of both p16INK4A and p14ARF expression, and immunoblot of SKMCs and HSMM cells in a study of stable ectopic expression of PAX3-FKHR in low passage primary human skeletal muscle cells. It is also suitable for microarray and indirect ELISA.

Monoclonal Anti-FKHR antibody has been used in:

• immunoblotting
• enzyme linked immunosorbent assay (ELISA)
• immunoprecipitation

Biochem/physiol Actions

FKHR plays a key regulatory roles in embryonic development, differentiation, apoptosis, and tumorigenesis. It was first identified at chromosomal breakpoints in human tumors and consequently linked to tumorigenesis. The activity of FKHR is regulated by growth factors via the PKB/PI3K pathway. The expression of FKHR is inhibited through phosphorylation by PKB. Within the nucleus, the dephosphorylated FKHRL1 induces expression of target genes such as Fas ligand, and triggers apoptosis. It is involved in apoptosis, glucose homeostasis, cell cycle regulation and as a nuclear receptor co factor. It has been found that FKHR also play a role in endometrial differentiation via the protein kinase A (PKA) pathway.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves