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Key Documents

F5875

Sigma-Aldrich

Ferredoxin from Spinacia oleracea (spinach)

buffered aqueous solution

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About This Item

CAS Number:
9040-09-9
MDL number:
MFCD00131049
UNSPSC Code:
12352202
NACRES:
NA.61

biological source

spinach (Spinacia oleracea)

Quality Level

200

form

buffered aqueous solution

concentration

1.0-3.0 mg/mL in 0.15 M Trizma buffer, pH 7.5 and NaCl (Frozen)

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

spinach ... SOVF(137530)

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Application

Ferredoxin has been used in studies of assimilation of inorganic nitrogen from nitrite or nitrate in the absence of ammonium. Ferredoxin is important in the electron transfer cascade required for this nitrogen assimilation.

Biochem/physiol Actions

Ferredoxins are iron-containing proteins that serve as electron-transfer catalysts in photosynthesis and nitrogen fixation.

Packaging

Package sizes are based on ferredoxin content.

Preparation Note

Partially purified by the method of Tagawa and Arnon, Nature, 195, 537 (1962).

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Basilio Zafrilla et al.
Biochemical Society transactions, 39(6), 1844-1848 (2011-11-23)
In the absence of ammonium, many organisms, including the halophilic archaeon Haloferax volcanii DS2 (DM3757), may assimilate inorganic nitrogen from nitrate or nitrite, using a ferredoxin-dependent assimilatory NO₃⁻/NO₂⁻ reductase pathway. The small acidic ferredoxin Hv-Fd plays an essential role in
Rosa M Martínez-Espinosa et al.
Biochimica et biophysica acta, 1623(1), 47-51 (2003-09-06)
Haloferax mediterranei is a halophilic archaeon that can grow in aerobic conditions with nitrate as sole nitrogen source. The electron donor in the aerobic nitrate reduction to ammonium was a ferredoxin. This ferredoxin has been purified and characterised. Air-oxidized H.
Rosa María Martínez-Espinosa et al.
FEMS microbiology letters, 277(1), 50-55 (2007-11-08)
Haloferax mediterranei is a halophilic archaeon that can grow using nitrate as the sole nitrogen source. A ferredoxin that serves as the physiological electron donor to the nitrate and nitrite reductases in this assimilatory process has been characterized. The ferredoxin
Pascal Belin et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(18), 7426-7431 (2009-05-07)
The gene encoding the cytochrome P450 CYP121 is essential for Mycobacterium tuberculosis. However, the CYP121 catalytic activity remains unknown. Here, we show that the cyclodipeptide cyclo(l-Tyr-l-Tyr) (cYY) binds to CYP121, and is efficiently converted into a single major product in

Protocols

Enzymatic Assay of Ferredoxin NADP Reductase (EC 1.18.12)

Enzymatic Assay of Ferredoxin NADP Reductase - To standardize a procedure for the enzymatic assay of Ferredoxin NADP Reductase (EC.1.18.12).

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