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Key Documents

C3545

Sigma-Aldrich

Monoclonal Anti-Calmodulin antibody produced in mouse

clone 6D4, ascites fluid

Synonym(s):

Anti-CALM3, Anti-calmodulin

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.44

€244.00


Availability
Ships within 2 business days(Orders outside of US, please allow an additional 1-2 weeks for delivery)

Request a Bulk Order

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

6D4, monoclonal

contains

15 mM sodium azide as preservative

species reactivity

chicken, Dictyostelium discoideum, bovine, rat

technique(s)

western blot: 1:2,000 using a bovine brain calmodulin preparation

isotype

IgG1

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General description

Calmodulin belongs to a family of structurally homologous Ca2+-binding proteins. CALM1 gene is located on human chromosome 14q32.11.
Calmodulin is a highly conserved 17kDa calcium binding protein found in all eukaryotic cells. It is a multifunctional molecule. Monoclonal anti-Calmodulin (mouse IgG1 isotype) is derived from the 6D4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with calmodulin, purified from Dictyostelium discoideum and conjugated to KLH.

Specificity

The antibody recognizes native and SDS-denatured calmodulin.
These antibodies are directed against different epitopes within the calmodulin molecule.

Immunogen

preparation of purified calmodulin from Dictyostelium discoideum conjugated to KLH.

Application

Monoclonal Anti-Calmodulin antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblot
  • dot blot
  • immunocytochemistry
  • immunoprecipitation
  • immunofluorescence[1]

Biochem/physiol Actions

Calmodulin is a ubiquitous molecule which can bind up to 4 calcium ions, regulating and mediating a wide variety of biochemical processes.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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    Association between the polymorphisms of CALM1 gene and osteoarthritis risk: a meta-analysis based on observational studies
    Yang H, et al.
    Bioscience Reports, 38(5), BSR20181128-BSR20181128 (2018)
    H C Lee et al.
    The Journal of biological chemistry, 270(16), 9060-9066 (1995-04-21)
    Cyclic ADP-ribose (cADPR) is emerging as an endogenous regulator of Ca2+-induced Ca2+ release (CICR), and we have recently demonstrated that its action is mediated by calmodulin (CaM) (Lee, H. C., Aarhus, R., Graeff, R., Gurnack, M. E., and Walseth, T.
    C J Pallen et al.
    The Journal of biological chemistry, 259(10), 6134-6141 (1984-05-25)
    Calcineurin possesses phosphatase activity towards both protein (Stewart, A.A., Ingebritsen , T.S., Manalan , A., Klee , C.B., and Cohen, P. (1982) FEBS Lett. 137, 80-84) and nonprotein substrates ( Pallen , C.J., and Wang, J.H. (1983) J. Biol. Chem.
    Maximal stimulation-induced in situ myosin light chain kinase activity is upregulated in fetal compared with adult ovine carotid arteries
    Injeti ER, et al.
    American Journal of Physiology. Heart and Circulatory Physiology, 295(6), H2289-H2298 (2008)
    The role of calmodulin and related proteins in plant cell function: an ever-thickening plot
    Bergey DR, et al.
    Springer Science Reviews, 2(1-2), 145-159 (2014)

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