C0493

Anti-Calcium Sensing Receptor antibody, Mouse monoclonal

clone HL1499 (6D4-3F4-6C4), purified from hybridoma cell culture

• Synonym(s): Anti-CAR, Anti-EIG8, Anti-FHH, Anti-FIH, Anti-GPRC2A, Anti-HHC, Anti-HHC1, Anti-HYPOC1, Anti-NSHPT, Anti-PCAR1, Anti-hCasR
• MDL number: MFCD02262635
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: HL1499 (6D4-3F4-6C4), monoclonal
• form: buffered aqueous solution
• species reactivity: rabbit, rat, mouse, human
• technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; indirect ELISA: suitable; western blot: 3-6 μg/mL using total extract from rat kidney
• isotype: IgG1
• UniProt accession no.: P41180
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CASR(846) ; mouse ... Casr(12374) ; rat ... Casr(24247)

Description

General description

The calcium sensing receptor (CASR) gene with 11 exons is mapped to human chromosome 3q13.33-q21.1. CASR is a member of the C family of G protein–coupled receptors and is mainly localized in the parathyroid gland and the thick ascending limb of the Henle loop. CASR is a 1078 amino acid protein.

Immunogen

peptide corresponding to amino-acids 15-29 at the extracellular N-terminus of calcium sensing receptor.

Application

Monoclonal Anti-Calcium Sensing Receptor antibody produced in mouse is suitable for the following applications:

• Indirect ELISA
• Immunoblotting
• Immunocytochemistry
• Immunohistochemistry (formalin-fixed, paraffin-embedded sections)
• Western blotting at a concentration of 3-6μg/mL using total extract from rat kidney

Monoclonal anti-calcium sensing receptor antibody produced in mouse has been used in western blotting and immunohistochemistry.

Biochem/physiol Actions

Calcium sensing receptor (CASR) regulates cell activity according to the extracellular calcium concentration. It also plays a major role in controlling parathyroid hormone (PTH) secretion. Thus, this protein can be considered as a potent target for therapeutic agents designed to modify parathyroid gland function. CASR has a role in key signal transduction pathways in several tissues such as parathyroid gland, kidney, gastrointestinal tract, placenta, pancreas and brain. Mutation in the gene is associated with autosomal dominant hypocalcemic, hypercalciuria and Bartter syndrome.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable