B9655

Anti-Avidin antibody, Mouse monoclonal, biotin conjugate

clone WC19.10, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-Avidin
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: biotin conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: WC19.10, monoclonal
• form: buffered aqueous solution
• technique(s): direct ELISA: suitable; dot blot: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Avidin is a glycoprotein (68 kDa) composed of four identical polypeptides chains isolated from egg whites.Monoclonal Anti-Avidin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

The antibody binds to the avidin molecule without interfering with the biotin-binding reaction. The product reacts specifically with egg white avidin, ExtrAvidin and their complexes formed with biotin. The antibody shows no cross-reaction with streptavidin.

Immunogen

Purified egg white avidin.

Application

Anti-Avidin-Biotin antibody, Mouse monoclonal has been used for fluorescence in situ hybridization (FISH) analysis of chromosomes X and 8 in lymphocytes isolated from women

Biochem/physiol Actions

Avidin has a high binding affinity for the vitamin, biotin. Biotin conjugated monoclonal anti-avidin increases specific avidin-biotin complex promoting the sensitivity of avidin-biotin immunoassays.

Physical form

Solution supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable