49290
β-Glucosidase from almonds
lyophilized, powder, ≥4 U/mg
Synonym(s):
β-D-Glucoside glucohydrolase
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About This Item
Recommended Products
form
powder
Quality Level
quality
lyophilized
specific activity
≥4 U/mg
mol wt
Mr ~135000
storage temp.
2-8°C
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Application
β-glucosidase is also used in the synthesis of glucosides and fucosides with various potential applications in pharmaceutical, cosmetic and detergent industries, hydrolytic removal of aglycone moiety from flavonoid and isoflavonoid glycosides, flavor enhancement of fruit juices and wine, and biosynthesis of oligosaccharides.
Biochem/physiol Actions
βglucosidase is involved in the hydrolysis of β-glycosidic bonds connecting carbohydrate residues in β-D-glycosides. They convert cellobiose and cellooligosaccharides produced by the endo and exoglucanases to glucose.
Unit Definition
1 U corresponds to the amount of enzyme which liberates 1 μmol glucose per minute at pH 5.0 and 37 °C (salicin as substrate)
Other Notes
Role of β-glucosidase in cellulose hydrolysis: Review; Enzymic hydrolysis of cellulose for fuel ethanol production by simultaneous saccharification and fermentation; Stereospecific attachment of carbohydrates to amino acid derivatives; Enzyme-catalyzed synthesis of alkyl β-D-glucosides in organic media
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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The transglycosylation from raffinose and lactose to Aloc-Ser-OMe is catalyzed respectively by alpha and beta galactosidases. Transglycosylation from cellobiose has been achieved with beta-glucosidase. The simplicity of the enzymatic synthesis, the stereospecificity of the condensations in one-pot reactions and the
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