05039

Agar

suitable for microbiology

• Synonym(s): Agar-agar, Gum agar
• Linear Formula: (C₁₂H₁₈O₉)_n
• CAS Number: 9002-18-0
• EC Number: 232-658-1
• MDL number: MFCD00081288
• UNSPSC Code: 41106212
• eCl@ss: 42040102
• NACRES: NA.74

Properties

• sterility: non-sterile
• Quality Level: 100
• form: powder
• shelf life: limited shelf life, expiry date on the label
• packaging: pkg of 50 g; pkg of 500 g
• storage condition: (Tightly closed)
• ign. residue: ≤5%
• loss: ≤12% loss on drying
• pH: 6-7.5 (1.5%after autoclaving)
• mp: 85-95 °C
• transition temp: gel point 33-37 °C (1.5% solution, after autoclaving)
• gel strength: >800 g/cm² (1.5% gel, Nikkan, after autoclaving)
• solubility: H₂O: 1.5% at 70 °C, clear to very faintly turbid, almost colorless
• application(s): agriculture; microbiology
• InChI: 1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1
• InChI key: GYYDPBCUIJTIBM-DYOGSRDZSA-N

Description

General description

Agar is widely used in microbiology as a solidifying agent in microbial culture media. It is a polysaccharide derived from seaweed and is primarily composed of agarose. Agar is a mostly inert substance, which makes it an ideal material for microbial culture media because it doesn’t interfere with the growth of microorganisms. Agar can solidify media, allowing microorganisms to grow in a solid environment, facilitating the formation of distinct colonies and selective isolation of specific microorganism. Agar is also used to prepare selective and differential media, which can provide additional nutrients or other chemical substances to promote or inhibit the growth of specific microorganisms selectively. Agar is sterilized by autoclaving and then poured into Petri dishesto cool and solidify, forming the surface for microbial growth.

Application

Agar is commonly used as a solidifying agent in many microbial culture media for the growth and identification of bacteria, yeasts, and molds from various pharmaceutical, environmental, and food and beverage samples.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves