11684302001
Roche
ABTS™ Solution
solution (ready-to-use), suitable for ELISA
Synonym(s):
ABTS™ Solution, ABTS
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352204
Recommended Products
form
solution (ready-to-use)
Quality Level
usage
sufficient for 1,500-3,000 assays (ELISA)
packaging
pkg of 3 × 100 mL
manufacturer/tradename
Roche
technique(s)
ELISA: suitable
storage temp.
2-8°C
Related Categories
General description
ABTS™ Solution is a ready to use substrate for peroxidase-driven indicator reactions. It contains 2,2-azino-di-[3-ethylbenzthiazoline sulfonate (6)] and H2O2 in glycin/citric acid buffer and is available in slightly green color.
Application
ABTS Solution is a chromogenic substrate for peroxidase in ELISA assays.
Biochem/physiol Actions
2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS™ acts a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS™ is a highly sensitive, specific and reproducible technique This solution is an excellent substrate for enzyme immunoassays with horseradish peroxidase as a marker enzyme.
Physical form
Reaction product:
Color: green
Evaluation: photometric (405nm)
Color: green
Evaluation: photometric (405nm)
Analysis Note
Absorption: ABTS absorption spectrum reference must always be measured and is determined by a spectrum. The Reference wavelength of 490 nm or higher should be selected due to no measureable absorption. The Reference absorption is usually automatically subtracted by the Plate Reader (background).
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
ABTS is a trademark of Roche
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Sylvia Herter et al.
Molecular cancer therapeutics, 12(10), 2031-2042 (2013-07-23)
We report the first preclinical in vitro and in vivo comparison of GA101 (obinutuzumab), a novel glycoengineered type II CD20 monoclonal antibody, with rituximab and ofatumumab, the two currently approved type I CD20 antibodies. The three antibodies were compared in
Yuanyuan Kuang et al.
EBioMedicine, 57, 102825-102825 (2020-06-20)
Numerous currently incurable human diseases have been causally linked to mutations in connexin (Cx) genes. In several instances, pathological mutations generate abnormally active Cx hemichannels, referred to also as "leaky" hemichannels. The goal of this study was to assay the
Gang Chen et al.
Biomolecules, 11(10) (2021-10-24)
Most recently, a technology termed TRIM-Away has allowed acute and rapid destruction of endogenous target proteins in cultured cells using specific antibodies and endogenous/exogenous tripartite motif 21 (TRIM21). However, the relatively large size of the full-size mAbs (150 kDa) results
H Matsuda et al.
The Japanese journal of experimental medicine, 54(3), 131-138 (1984-06-01)
A micro-technique of enzyme-linked immunosorbent assay (ELISA) using ABTS, 2,2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as a substrate for horseradish peroxidase (HRP) conjugate was studied. In a comparative study among 4 substrates, namely; 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT) and ABTS, for
Monica Musiani et al.
Nature protocols, 2(10), 2502-2510 (2007-10-20)
PCR is an established technique providing rapid and highly productive amplification of specific DNA sequences. The demand for equally rapid, sensitive and objective methods to achieve detection of PCR products has led to the coupling of PCR with ELISA. PCR-ELISA
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service