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Key Documents

MAB2500

Sigma-Aldrich

Anti-Collagen Type VII Antibody, clone 32,-VII

Chemicon®, from mouse

Synonym(s):

Anti-EBD1, Anti-EBDCT, Anti-EBR1, Anti-NDNC8

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100
300

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

32, monoclonal
mAb-VII, monoclonal

species reactivity

bovine, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_000094.3

UniProt accession no.

Q02388

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... COL7A1(1294)

Specificity

Monoclonal antibody MAB2500 recognizes human and bovine Type VII Collagen.

Application

Anti-Collagen Type VII Antibody, clone 32, -VII detects level of Collagen Type VII & has been published & validated for use in ELISA.
ELISA

IH on unfixed, weakly fixed tissue sections.

Western Blot Analysis: A 1:1000 dilution from a representative lot detected Type VII collagen in HeLa cells.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Physical form

Format: Purified
Liquid at 1 mg/mL in 20 mM phosphate buffer, 250 mM NaCl, pH 7.6, containing 0.1% sodium azide.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Markus Kretz et al.
Nature, 493(7431), 231-235 (2012-12-04)
Several of the thousands of human long non-coding RNAs (lncRNAs) have been functionally characterized; however, potential roles for lncRNAs in somatic tissue differentiation remain poorly understood. Here we show that a 3.7-kilobase lncRNA, terminal differentiation-induced ncRNA (TINCR), controls human epidermal
Adam J Rubin et al.
Nature genetics, 49(10), 1522-1528 (2017-08-15)
Chromosome conformation is an important feature of metazoan gene regulation; however, enhancer-promoter contact remodeling during cellular differentiation remains poorly understood. To address this, genome-wide promoter capture Hi-C (CHi-C) was performed during epidermal differentiation. Two classes of enhancer-promoter contacts associated with
The development and characterization of an in vitro model of psoriasis.
Barker, CL; McHale, MT; Gillies, AK; Waller, J; Pearce, DM; Osborne, J; Hutchinson et al.
The Journal of Investigative Dermatology null

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