MAB2014
Anti-OB-Cadherin Antibody, extracellular domain, clone 16A
clone 16A, Chemicon®, from mouse
Synonym(s):
Cadherin-11
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
16A, monoclonal
species reactivity
rat, human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CDH11(1009)
General description
Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is based on the fact that migration of mesenchymal cells is facilitated when cadherin-11 is highly expressed.
Specificity
Reacts with the extracellular domain of human cadherin-11.
Immunogen
Epitope: extracellular domain
Fusion protein corresponding to extracellular domain of human OB-cadherin coupled to GST.
Application
Detect OB-Cadherin using this Anti-OB-Cadherin Antibody, extracellular domain, clone 16A validated for use in WB, IC, IH.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
Western blot: 1:25 - 1:250
Immunohistochemistry: Frozen tissue sections. 1:25 - 1:50 dilution using avidin-biotinylated horseradish peroxidase complex (ABC) as detection protocol.
Immunocytochemistry: Methanol and paraformaldehyde-fixed cells
Optimal working dilutions must be determined by the end user.I/
Immunohistochemistry: Frozen tissue sections. 1:25 - 1:50 dilution using avidin-biotinylated horseradish peroxidase complex (ABC) as detection protocol.
Immunocytochemistry: Methanol and paraformaldehyde-fixed cells
Optimal working dilutions must be determined by the end user.I/
Physical form
Affinity purified immuoglobulin. Liquid in PBS containing 0.09% sodium azide.
Format: Purified
Storage and Stability
1 year at 4°C from date of shipment
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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