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MAB19562X

Sigma-Aldrich

Anti-Laminin-5 Antibody, clone D4B5, Alexa Fluor 488 conjugated

clone D4B5, Chemicon®, from mouse

Synonym(s):

Anti-B2T, Anti-CSF, Anti-EBR2, Anti-EBR2A, Anti-LAMB2T, Anti-LAMNB2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Pricing and availability is not currently available.

biological source

mouse

Quality Level

conjugate

ALEXA FLUOR 488

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

D4B5, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable

isotype

IgG1

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biological source

mouse

biological source

mouse

biological source

mouse

biological source

rat

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

mouse

conjugate

ALEXA FLUOR 488

conjugate

-

conjugate

-

conjugate

ALEXA FLUOR 488

clone

D4B5, monoclonal

clone

D4B5, monoclonal

clone

P3E4, monoclonal

clone

13A4, monoclonal

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified antibody

General description

Laminins are basement membrane, extracellular matrix glycoproteins that are composed of alpha, beta and gamma heterotrimeric chains. Different combinations of five alpha chains, three beta chains, and two gamma chains form at least 12 laminin isoforms. Laminin-5 (LN5), which consists of laminin alpha3, beta3 and gamma2 chains, is a highly conserved component of anchoring filaments underlying the hemidesmosome structure of epidermal keratinocytes. Mutations or deletion in the LN5 genes (LAMA3, LAMB3, and LAMC2) is associated with epidermolysis bullosa, a lethal skin blistering disease. LN5 may stabilize the epidermal/dermal junction of skin through binding with integrins alpha3/beta1, alpha6/beta4 and type VII collagen and has a critical role in the anchorage and locomotion of a wide variety of cell types, including epithelial cells, fibroblasts, neurons and leukocytes. Compared to fibronectin, collagen, or vitronectin, cells of epithelial origin will adhere to Laminin-5 faster and will spread to a larger extent. Recently, Laminin-5 is attracting attention as a substrate for carcinoma cells that may stimulate migration, since it has been detected at the leading edge of invasive cancer tissue in several types of human carcinomas (Niki, et al., 2002, Lohi, et al., 2001).

Specificity

Monoclonal antibody MAB19562 was developed against amino acid residues 382-608 of human laminin gamma2 chain (III domain) expressed as a GST fusion protein. The antibody recognizes the 150 kDa (precursor form) and 105 kDa (mature form) chain proteins of Laminin-5. No reactivity is expected with other laminin types, as the gamma2 chain of laminin is unique to laminin-5.

Immunogen

Amino acid residues 382-608 of human laminin gamma2 chain (III domain) expressed as a GST fusion protein.
Epitope: amino acids 382-608

Application

Immunocytochemistry: 2 μg/mL
Flow cytometry
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins
This Anti-Laminin-5 Antibody, clone D4B5, Alexa Fluor 488 conjugated is validated for use in FC, IC for the detection of Laminin-5.

Physical form

Purified immunoglobulin conjugated to Alexa Fluor 488. Liquid in PBS containing 1% BSA, 0.05% Tween-20 and 0.05% sodium azide.

Storage and Stability

Maintain at 2°-8°C for up to 12 months from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Alexa Fluor is a registered trademark of Molecular Probes, Inc.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


  • Certificates of Analysis (COA)

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