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Key Documents

MAB1910

Sigma-Aldrich

Anti-Collagen Type IV α 2 Chain Antibody, clone 23IIC3

ascites fluid, clone 23IIC3, Chemicon®

Synonym(s):

Anti-BSVD2, Anti-ICH, Anti-POREN2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

23IIC3, monoclonal

species reactivity

monkey, human, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... COL4A2(1284)

Specificity

Recognizes Human type IV collagen, alpha-2 chains.

Immunogen

Human amniotic type IV collagen

Application

Detect Collagen Type IV α 2 Chain using this Anti-Collagen Type IV α 2 Chain Antibody, clone 23IIC3 validated for use in ELISA, IH(P) & WB.
Immunohistochemistry: 1:500, acetone fixed, frozen tissue recommended. Weak reactivity on on formalin fixed, paraffin sections (1:100) after treatment with 1% trypsin (20 minutes, room temperature). Or alternatively, 4% PFA fixed, HEIR treated tissue has worked in monkey (Qin, 2003) at 1:200 dilution.

Western blot: 1:500, 170 kDa.

ELISA at 1:200

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Target description

170 kDa

Physical form

Liquid.
Unpurified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Control: Kidney, muscle, tendon spleen tissue
Negative Control: Neurons/glia

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Edward R Chu et al.
Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics, 30(2-3), 191-201 (2014-02-13)
We have developed a tissue-based model of the human trabecular meshwork (TM) using viable postmortem corneoscleral donor tissue. Two-photon microscopy is used to optically section and image deep in the tissue to analyze cells and extracellular matrix (ECM) within the
Peter Trosan et al.
PloS one, 13(3), e0194820-e0194820 (2018-03-28)
The human amniotic membrane (HAM) is widely used for its wound healing effect in clinical practice, as a feeder for the cell cultivation, or a source of cells to be used in cell therapy. The aim of this study was
D Ohlund et al.
British journal of cancer, 101(1), 91-97 (2009-06-06)
Pancreas cancer is a dreaded disease with high mortality, despite progress in surgical and oncological treatments in recent years. The field is hampered by a lack of good prognostic and predictive tumour biomarkers to be used during follow-up of patients.

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