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Key Documents

GF343

Sigma-Aldrich

PDGF-AA,
Human Recombinant Animal Free

Synonym(s):

PDGFA, PDGF-1, PDGF-A, PDGF1

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.77

Pricing and availability is not currently available.

impurities

<0.1 μg/mg protein endotoxin (<1 EU/μg)

Quality Level

UniProt accession no.

shipped in

dry ice

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This Item
GF310SRP3228GF142
impurities

<0.1 μg/mg protein endotoxin (<1 EU/μg)

impurities

<0.1 μg/mg protein endotoxin (<1EU/μg)

impurities

<0.1 EU/μg endotoxin, tested

impurities

-

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

Quality Level

100

Quality Level

100

Quality Level

-

Quality Level

100

General description

PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR- α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGFBB and PDGF-AB.
Recombinant human PDGF-AA is a 28.5 kDa disulfide-linked homodimer of two A chains (250 total amino acids).
Product Source: Protein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.

Application

Research Category
Stem Cell Research
Research Sub Category
Growth Factors & Receptors

Quality

Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.

Physical form

Product is presented in 10mM Acetic Acid and is filtered through a 0.2 micron filter before lyophilization.

Storage and Stability

Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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