ABN78A4

Anti-NeuN (rabbit) Antibody, Alexa Fluor^™ 488 Conjugate

from rabbit, ALEXA FLUOR^™ 488

• Synonym(s): NEUronal Nuclei, Neuronal Nuclei
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: ALEXA FLUOR^™ 488
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat, mouse
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Rbfox3(52897) ; rat ... Rbfox3(287847)

Description

General description

ABN78 is a rabbit polyclonal version of the Anti-NeuN, clone A60 (MAB377), NeuN is a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

Specificity

The unconjugated rabbit Anti-NeuN (Catalog No. ABN78) has been shown to work in human.

This antibody recognizes NeuN at the N-terminus.

Immunogen

Epitope: N-terminus

GST-tagged recombinant protein corresponding to mouse NeuN.

Application

Detect NeuN (rabbit) using this Anti-NeuN (rabbit) Antibody, Alexa Fluor 488 Conjugate validated for use in IC, IH.

Immunocytochemistry analysis: A 1:100 dilution from a representative lot detected NeuN in rat E18 primary cortex cells.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Immunohistochemistry in adult mouse brain tissue.

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in adult mouse brain tissue.

Target description

The unconjugated parent antibody (Cat. No. ABN78) has an observed molecular weight at ~46/48 kDa An uncharacterized band appears at ~62 kDa in some lysates.

Physical form

Affinity purified

Purified rabbit polyclonal conjugated to Alexa Fluor^™ 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Adult mouse brain tissue

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable