20-188
RIPA Lysis Buffer, 10X
100 mL RIPA Lysis Buffer, 10X for Immunoprecipitation & Western Blotting.
Synonym(s):
lysis buffer
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About This Item
UNSPSC Code:
41116010
eCl@ss:
42029053
NACRES:
NB.22
form
liquid
packaging
pkg of 100 mL
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
shipped in
ambient
General description
Radioimmunoprecipitation assay (RIPA) Buffer aids in cell lysis and solubilization of proteins from both adherent and suspension-cultured mammalian cells. In addition, this buffer helps in relevant protein-protein interactions studies by reducing non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur.
Application
RIPA Lysis Buffer, 10X has been used for cell lysis during the sample preparation for immunoprecipitation and western blot.
Physical form
0.5M Tris-HCl, pH 7.4, 1.5M NaCl, 2.5% deoxycholic acid, 10% NP-40, 10mM EDTA.
Storage and Stability
6 months at room temperature
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Certificates of Analysis (COA)
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