17-295

Chromatin Immunoprecipitation (ChIP) Assay Kit

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein A agarose beads.

• Synonym(s): Agarose ChIP Kit, ChIP, Chromatin enrichment, chromatin immunoprecipitation
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.41

Properties

• Quality Level: 100
• manufacturer/tradename: EZ-ChIP
• technique(s): immunoprecipitation (IP): suitable
• shipped in: wet ice

Description

General description

For use to enrich for specific regions of chromatin that are immunoprecipitated with a specific antibody for a chromatin protein. Works with all eukaryotic cell types. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR detection is recommended.

Packaging

Kit capacity: 22 Assays

Components

Protein A agarose/Salmon Sperm DNA (Cat.# 16-157)

All necessary buffers

Quality

Routinely evaluated using anti-acetyl Histone H4 polyclonal antibody (06-866) to immunoprecipitate acetylated histone H4. Subsequent immunoblot analysis was done using the same antibody.

Physical form

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319,H412
• Precautionary Statements: P264 - P273 - P280 - P305 + P351 + P338 - P337 + P313 - P501
• Hazard Classifications: Aquatic Chronic 3 - Eye Irrit. 2
• Storage Class Code: 12 - Non Combustible Liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable