09-838
Anti-Histone H3.3 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Histone H3.3
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
technique(s)
ChIP: suitable
dot blot: suitable
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... H3F3B(3021)
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone variant H3.3 is typically enriched in active chromatin.
Specificity
Broad species cross-reactivity expected based on 100% sequence homology.
Immunogen
KLH-conjugated linear peptide corresponding to human Histone H3.3.
Application
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to detect Histone H3.3 in HeLa cells.
Image courtesy of Simon Elsässer of David Allis′ Laboratory, Rockefeller University, New York.
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP.
Image courtesy of Simon Elsässer of David Allis′ Laboratory, Rockefeller University, New York.
Dot Blot Analysis: A representative lot was used by an independent laboratory in DB.
Reported by Simon Elsässer, Laboratory of Dr. David Allis, Rockefeller University, New York.
Image courtesy of Simon Elsässer of David Allis′ Laboratory, Rockefeller University, New York.
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP.
Image courtesy of Simon Elsässer of David Allis′ Laboratory, Rockefeller University, New York.
Dot Blot Analysis: A representative lot was used by an independent laboratory in DB.
Reported by Simon Elsässer, Laboratory of Dr. David Allis, Rockefeller University, New York.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-Histone H3.3 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, DB, ChIP to detect Histone H3.3 also known as Histone H3.3.
Quality
Evaluated by Western Blot in HeLa acid extract.
Western Blot Analysis: 1 µg/mL of this antibody detected Histone H3.3 on 10 µg of HeLa acid extract.
Western Blot Analysis: 1 µg/mL of this antibody detected Histone H3.3 on 10 µg of HeLa acid extract.
Target description
~17 kDa observed
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa acid extract
HeLa acid extract
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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The octamer is the major form of CENP-A nucleosomes at human centromeres.
Hasson, D; Panchenko, T; Salimian, KJ; Salman, MU; Sekulic, N; Alonso, A; Warburton, PE; Black, BE
Nature Structural and Molecular Biology null
Transcription-coupled recruitment of human CHD1 and CHD2 influences chromatin accessibility and histone H3 and H3.3 occupancy at active chromatin regions.
Siggens, L; Cordeddu, L; Ronnerblad, M; Lennartsson, A; Ekwall, K
Epigenetics & Chromatin null
DAXX-dependent supply of soluble (H3.3-H4) dimers to PML bodies pending deposition into chromatin.
Delbarre, E; Ivanauskiene, K; Kuntziger, T; Collas, P
Genome Research null
Alyshia Newhart et al.
The Journal of biological chemistry, 288(27), 19882-19899 (2013-05-22)
Unlike the core histones, which are incorporated into nucleosomes concomitant with DNA replication, histone H3.3 is synthesized throughout the cell cycle and utilized for replication-independent (RI) chromatin assembly. The RI incorporation of H3.3 into nucleosomes is highly conserved and occurs
Hsiao P J Voon et al.
Cell reports, 11(3), 405-418 (2015-04-14)
Histone H3.3 is a replication-independent histone variant, which replaces histones that are turned over throughout the entire cell cycle. H3.3 deposition at euchromatin is dependent on HIRA, whereas ATRX/Daxx deposits H3.3 at pericentric heterochromatin and telomeres. The role of H3.3
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