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Key Documents

07-540

Sigma-Aldrich

Anti-acetyl-Histone H3 (Lys36) Antibody

serum, Upstate®

Synonym(s):

H3K36Ac, Histone H3 (acetyl K36)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

multiplexing: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

acetylation (Lys36)

Gene Information

human ... H3F3B(3021)

Specificity

Broad species cross reactivity is expected
Recognizes Histone H3 when acetylated on Lys36

Immunogen

KLH-conjugated, synthetic peptide containing the sequence ...GV[AcK]KP... in which [AcK] corresponds to acetyl-lysine 36 of human histone H3.

Application

Suggested dilutions:
Western Blot: 1:5000 - 1:20,000

ChIP Analysis: A representative lot was used by an independent laboratory for ChIP (Gatta, R., et al. (2011). Epigenetics. 6(4):526-534.)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-acetyl-Histone H3 (Lys36) Antibody (Rabbit Polyclonal Antibody) validated in WB, Mplex to detect acetyl-Histone H3 (Lys36) also known as H3K36Ac, Histone H3 (acetyl K36).

Quality

Routinely evaluated by immunoblot.

Target description

~17kDa

Physical form

100μl of rabbit antiserum containing 0.05% sodium azide and 30% glycerol.
Antiserum

Storage and Stability

2 years at -20°C from date of shipment

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cluster analysis reveals differential transcript profiles associated with resistance training-induced human skeletal muscle hypertrophy.
Thalacker-Mercer, A; Stec, M; Cui, X; Cross, J; Windham, S; Bamman, M
Physiological Genomics null
Raffaella Gatta et al.
Epigenetics, 6(4), 526-534 (2011-02-10)
Histones post-translational modifications (PTMs) are crucial for transcriptional control, defining positive and negative chromatin territories. We previously described an extensive methylation-acetylation switch on cell cycle promoters using a single nucleosome ChIP assay. A key issue is how PTMs are locally
Anton Eberharter et al.
EMBO reports, 3(3), 224-229 (2002-03-08)
The organization of eukaryotic chromatin has a major impact on all nuclear processes involving DNA substrates. Gene expression is affected by the positioning of individual nucleosomes relative to regulatory sequence elements, by the folding of the nucleosomal fiber into higher-order
Jihye Jung et al.
Frontiers in microbiology, 11, 1978-1978 (2020-09-26)
Sound vibration (SV) is one of the several environmental stimuli that induce physiological changes in plants including changes in plant immunity. Immune activation is a complicated process involving epigenetic modifications, however, SV-induced epigenetic modifications remain unexplored. Here, we performed an
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Thapa, A; Morris, L; Xu, J; Ma, H; Michalakis, S; Biel, M; Ding, XQ
The Journal of Biological Chemistry null

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