05-632

Anti-14-3-3σ Antibody, clone CS112-2A8

clone CS112-2A8, Upstate^®, from mouse

• Synonym(s): Stratifin
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: CS112-2A8, monoclonal
• species reactivity: mouse, human, rat
• manufacturer/tradename: Upstate^®
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NM_018754
• UniProt accession no.: P31947
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... SFN(2810) ; mouse ... Sfn(55948) ; rat ... Sfn(313017)

Description

Specificity

Recognizes 14-3-3σ, does not cross-react with other 14-3-3 isoforms.

Immunogen

GST fusion protein corresponding to full-length human 14-3-3 sigma

Application

Detect 14-3-3σ using this Anti-14-3-3σ Antibody, clone CS112-2A8 validated for use in IP & WB.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Quality

routinely evaluated by immunoblot on RIPA lysates of EGF-stimulated A431 cells

Target description

30 kDa

Physical form

Format: Purified

Protein G Purified

Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable