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Key Documents

04-085

Sigma-Aldrich

Anti-Ago family Antibody

Upstate®, from mouse

Synonym(s):

argonaute family, piwi

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... AGO1(26523)

Specificity

Ago family proteins

Immunogen

Synthetic peptide PKIDVYHYEVDIKPDKCPRRVNREVV

Application

Suggested dilution:
Western Blot: 1:500
Research Category
Protein Trafficking
Research Sub Category
RNA Metabolism & Binding Proteins

RNA Binding Protein (RBP)
Use Anti-Ago family Antibody (Mouse Monoclonal Antibody) validated in WB to detect Ago family also known as argonaute family PIWI.

Quality

Routinely evaluated by immunoblot.

Target description

~100 kDa

Physical form

100 μg of Protein A purified mouse monoclonal IgG1κ in 100μl of 1X PBS, pH 7.0, 0.1% Azide.
Format: Purified
Protein A purified

Storage and Stability

2 years at -20°C from date of shipment

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi
Yigit, Erbay, et al
Cell, 127, 747-757 (2006)
Wibke J Meyer et al.
PLoS genetics, 2(8), e134-e134 (2006-08-29)
Argonaute proteins are essential components of the molecular machinery that drives RNA silencing. In Drosophila, different members of the Argonaute family of proteins have been assigned to distinct RNA silencing pathways. While Ago1 is required for microRNA function, Ago2 is
Differential remodeling of mono- and trimethylated H3K27 during porcine embryo development.
Park KE, Magnani L, Cabot RA
Molecular Reproduction and Development null
Interactions between the RNA interference effector protein Ago1 and 14-3-3 proteins: consequences for cell cycle progression
Stoica, Cezar, et al
The Journal of Biological Chemistry, 281, 37646-37651 (2006)

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