QBD10774

NHS-dPEG^®₂₄-biotin

• Synonym(s): Polyethylene glycol
• Empirical Formula (Hill Notation): C₆₅H₁₂₀N₄O₃₀S
• Molecular Weight: 1469.72
• UNSPSC Code: 51171641
• PubChem Substance ID: 329823948
• NACRES: NA.22

Properties

• Assay: >90%
• form: solid or viscous liquid
• reaction suitability: reaction type: Biotinylations; reactivity: amine reactive; reagent type: cross-linking reagent
• polymer architecture: shape: linear; functionality: monofunctional
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: O=C(CCCCC1SCC(N2)C1NC2=O)NCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCC(ON3C(CCC3=O)=O)=O

Description

General description

NHS-dPEG^®₂₄-biotin is a cross-linking reagent used in biotinylation reactions. The N-hydroxysuccinimidyl (NHS) moiety of NHS-dPEG^®₂₄-biotin forms a stable amide bond with free primary amines, such as the amines on the side chain of lysine. The biotin moiety is used in a variety of applications, including labeling, supramolecular building, affinity chromatography, pull-down assays, and the production of biotinylated antibodies.

Features and Benefits

NHS-dPEG^®₂₄-biotin is used extensively to label molecules that contain a free amine with biotin. The single molecular weight NHS-dPEG^®₂₄-biotin compound has a long spacer of precise length (97.7 Å) between the biotin moiety and the labeled molecule. This dPEG^® spacer imparts a high degree of water solubility to the biotin label. For biotinylated peptides and proteins, the dPEG^® spacer dramatically reduces aggregation and precipitation of the modified molecule and reduces non-specific binding of the molecule, both of which are common problems with molecules that have been biotinylated with the widely used NHS-LC-biotin.

Legal Information

Products Protected under U.S. Patents # 7,888,536 B2

dPEG is a registered trademark of Quanta BioDesign

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable