330000

Rose bengal

Dye content 95 %, ≥96% (HPLC)

• Synonym(s): 4,5,6,7-Tetrachloro-2′,4′,5′,7′-tetraiodofluorescein disodium salt, Acid Red 94, Bengal Rose B sodium salt, Rose Bengal sodium salt
• Empirical Formula (Hill Notation): C₂₀H₂Cl₄I₄Na₂O₅
• CAS Number: 632-69-9
• Molecular Weight: 1017.64
• Beilstein: 3645857
• EC Number: 211-183-3
• MDL number: MFCD00005043
• UNSPSC Code: 12171500
• PubChem Substance ID: 24859827
• NACRES: NA.47

Properties

• Assay: ≥96% (HPLC)
• form: solid
• composition: Dye content, 95%
• technique(s): titration: suitable
• color: red to brown
• ε (extinction coefficient): ≥ 3000 at 350-354 nm; ≥12000 at 311-315 nm; ≥32000 at 262-266 nm; ≥32000 at 513-517 nm; ≥98000 at 546-550 nm at 0.04 g/L (H2O + 1ml 1% Na2CO3)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[O-]C(=O)c1c(Cl)c(Cl)c(Cl)c(Cl)c1C2=C3C=C(I)C(=O)C(I)=C3Oc4c(I)c([O-])c(I)cc24
• InChI: 1S/C20H4Cl4I4O5.2Na/c21-10-8(9(20(31)32)11(22)13(24)12(10)23)7-3-1-5(25)16(29)14(27)18(3)33-19-4(7)2-6(26)17(30)15(19)28;;/h1-2,29H,(H,31,32);;/q;2*+1/p-2
• InChI key: UWBXIFCTIZXXLS-UHFFFAOYSA-L

Description

General description

Rose Bengal is a red or rose color dye and is anionic in nature. It is a 4,5,6,7-tetrachloro 2′,4′,5′,7′-tetraiodo derivative of fluorescein. It is widely used to detect damage to the ocular surface epithelium in ocular surface diseases such as dry eye and herpetic keratitis. Rose Bengal has mild antiviral potency.

Rose Bengal is used to stain dead and degenerating cells and mucus.

Application

Rose Bengal has been used to evaluate acrosome integrity. It has also been used to initiate photothrombotic occlusion in blood.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves