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RAB0145

Sigma-Aldrich

Human Dkk-3 ELISA Kit

for serum, plasma, cell culture supernatants and urine

Synonym(s):

Dickkopf-related protein 3, Dkk-3

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type plasma
sample type urine
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 2.74-2000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... DKK3(27122)

General description

The Human Dickkopf homolog 3 (DKK3) enzyme-linked immunosorbent assay (ELISA) kit is an in vitro enzyme-linked immunosorbent assay used for the quantitative measurement of human DKK-3 in serum, plasma, cell culture supernatants, and urine. Dickkopf homolog 3 (DKK3) protein belongs to the Dkk family. The DKK3 gene acts as a tumor suppressor gene and is suppressed in several human cancers. This gene is mapped on the human chromosome at 11p15.3.

Immunogen

Recombinant Human DKK3

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human Dkk-3 ELISA Kit has been used to evaluate Dickkopf homolog 3 (DKK3) from conditioned media. It has also been used to determine DKK3 levels in urine samples.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jun Yu et al.
Cancer, 115(1), 49-60 (2008-12-04)
Abnormal activation of the Wnt/beta-catenin signaling pathway is common and critical in the pathogenesis of digestive cancers. In this study, the authors investigated the promoter methylation of the dickkopf homolog 3 gene Dkk-3 in these cancers and its prognostic significance
Kenta Torigoe et al.
Medicina (Kaunas, Lithuania), 57(6) (2021-07-03)
Background and Objectives: Urinary levels of dickkopf-3 (DKK-3) are associated with poor renal survival in patients with non-dialytic chronic kidney disease. However, it remains unknown whether urinary DKK-3 levels can predict residual renal function (RRF) decline in patients undergoing peritoneal
Shuang Zhao et al.
Frontiers in oncology, 10, 600322-600322 (2021-01-12)
Dkk3 has been discovered during comparison of immortalized and parental cells. Its expression has been shown to reduce colony formation and induce apoptosis of cancer cells, acting as a tumor suppressor. Herein, we demonstrate that Dkk3 overexpression or protein treatment
Francesca Valdora et al.
International journal of molecular sciences, 14(4), 7492-7505 (2013-04-10)
Medulloblastoma (MB) is a malignant pediatric brain tumor arising in the cerebellum consisting of four distinct subgroups: WNT, SHH, Group 3 and Group 4, which exhibit different molecular phenotypes. We studied the expression of Dickkopf (DKK) 1-4 family genes, inhibitors
Kazuyasu Kobayashi et al.
Gene, 282(1-2), 151-158 (2002-01-30)
The human REIC gene is a recently found mortalization-related gene and a candidate tumor suppressor gene expression of which is largely attenuated in many immortalized and tumor-derived cell lines (Biochem. Biophys. Res. Commun. 268 (2000) 20-24). To gain insight into
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