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N7265

Sigma-Aldrich

Nitrate Reductase (NAD[P]H) from Aspergillus niger

lyophilized powder, ≥300 units/g solid

Synonym(s):

NAD(P)H:Nitrate oxidoreductase

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About This Item

CAS Number:
9029-27-0
Enzyme Commission number:
1.7.1.2 (BRENDA, IUBMB)
MDL number:
MFCD00131725
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.

form

lyophilized powder

Quality Level

200

specific activity

≥300 units/g solid

shipped in

wet ice

storage temp.

−20°C

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General description

Nitrate reductase (NAD[P]H) is expressed in variety of organisms including bacteria, fungi, algae, and higher plants which have capacity to use nitrate as their sole source of nitrogen.Experimental studies show that nitrate reductase (NAD[P]H) is 180,000Daltons.[1]

Biochem/physiol Actions

Nitrate Reductase (NAD[P]H) catalyzes the reduction of nitrate to nitrite. In addition, it also catalyzes the reduction of NADPH-cytochrome c, FADH2-nitrate. Nitrate reductase also has reduced methyl viologen-nitrate reductase activities.[1]

Caution

light and moisture sensitive

Unit Definition

One unit will reduce 1.0 μmole of nitrate per min in the presence of β-NADPH at pH 7.5 at 25 °C.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000347507
0000351286
0000347510
0000347509
0000331250

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P C Allen
Poultry science, 76(6), 810-813 (1997-06-01)
The objective of this study was to gather evidence for production of nitric oxide (NO) during a primary infection with the protozoan parasite Eimeria tenella, which carries out its life cycle in the ceca of chickens. Relationships of plasma levels
M A de la Rosa et al.
European journal of biochemistry, 106(1), 249-256 (1980-05-01)
Assimilatory nitrate reductase [NAD(P)H] (EC 1.6.6.2) from Ankistrodesmus braunii has been purified to homogeneity by a simple procedure that utilizes as the main step affinity chromatography on Blue-Sepharose. The best enzyme preparation has a specific activity of 61.25 units/mg protein.
J Miyazaki et al.
Molecular & general genetics : MGG, 228(3), 329-334 (1991-09-01)
Barley (Hordeum vulgare L.) has both NADH-specific and NAD(P)H-bispecific nitrate reductases. Genomic and cDNA clones of the NADH nitrate reductase have been sequenced. In this study, a genomic clone (pMJ4.1) of a second type of nitrate reductase was isolated from
W H Campbell
Cellular and molecular life sciences : CMLS, 58(2), 194-204 (2001-04-06)
Pyridine nucleotide-dependent nitrate reductases (NRs; EC 1.6.6.1-3) are molybdenum-containing enzymes found in eukaryotic organisms which assimilate nitrate. NR is a homodimer with an approximately 100 kDa polypeptide which folds into stable domains housing each of the enzyme's redox cofactors--FAD, heme-Fe
P C Allen et al.
Poultry science, 76(6), 822-827 (1997-06-01)
Two experiments were conducted to determine effects of diets containing n-3 fatty acids (n-3FA) from whole as well as ground flaxseed on the performance of broilers during coccidia infections. Diets were fed from 1 d of age through 3 wk
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Questions

1–2 of 2 Questions  

  1. How much does 2units weigh?

    1 answer

    1. The amount of solid provided will depend on the enzymatic activity reported on the lot specific Certificate of Analysis. For example, lot SLCR1079 has the enzymatic activity value of 481 units per gram, thus the 2 unit package size would contain roughly 4.16 mg of solid.

      Please view the sample Certificate of Analysis below to see the enzymatic activity:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/N7265-BULKSLCR1079.pdf

      Helpful?

  2. Guten Tag, benötige ich für die Reaktion denn nicht auch das FAD ?

    1 answer

    1. FAD, Flavin Adenine Dinucleotide, is a component used in determining the activity of Nitrate Reductase. See the link below to review the enzymatic assay protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/257/754/n7265enz.pdf

      Helpful?

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