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MSRT1

Sigma-Aldrich

MS RT Calibration Mix

Proteomics Retention Time Standard for LC-MS

Synonym(s):

Proteomics Retention Time Standard for LC-MS

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.24

form

ready-to-use solution

Quality Level

200

quality

Proteomics Retention Time Standard for LC-MS

analyte chemical class(es)

amino acids, peptides, proteins

technique(s)

HPLC: suitable
LC/MS: suitable

application(s)

food and beverages

format

multi-component solution

storage temp.

−20°C

General description

MSRT1 is an injection-ready standard, and can serve for quick tests of LC-MS platform performance, comparison of LC gradients and columns, monitoring column and system changes. MSRT1 can be used to predict retention times of peptides across LC-MS platforms. MSRT1 is also applicable to multiple reaction monitoring / selected reaction monitoring (MRM / SRM) analysis.

Components:
Each vial contains various amounts of 14 isotopically labeled synthetic peptides dried via vacuum centrifugation. Each peptide is labeled with either a [13C6,15N2] Lysine (+8 Da), a [13C6,15N4] Arginine (+10 Da), or a [13C6,15N] Leucine (+7 Da).

Application

MS RT Calibration Mix has been used as a reference for iRT (relative retention times) calculations after LC-MS (liquid chromatography–mass spectrometry)/MS analysis. It has been used to check the reliability and specificity of ETD-HCD (electron transfer dissociation-high-energy collisional dissociation) MS3 (mass spectrometry) method for leucine/isoleucine discrimination.
The MS RT Calibration Mix is a LC-MS platform standard intended to test properties such as LC resolution, peptide elution profiles, and retention time prediction. The peptides in MSRT1 are designed to span the normal elution profile of complex proteomic samples. The peptides have been added in various amounts, based on electrospray response, to have relatively similar response and be readily observed.

MSRT1 is an injection-ready standard, and can serve for quick tests of LC-MS platform performance, comparison of LC gradients and columns, monitoring column and system changes. MSRT1 can be used to predict retention times of peptides across LC-MS platforms. MSRT1 is also applicable to multiple reaction monitoring / selected reaction monitoring (MRM / SRM) analysis.

Features and Benefits

General

Complexity

  • Defined mixture gives confidence in your instruments analysis

Easily visualized
  • Distinct LC-MS peak profile of peptides, readily separated with similar responses
  • iRT Table (xls)

Injection-ready
  • Decreases prep time

Qualitative:
  • Defined mixture of 14 stable isotope labeled (SIL) peptides

Quantitative:
MRM settings provided (Skyline, xls)

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Legal Information

This product is licensed under U.S. Patent No. 7,396,688 and foreign counterparts from E. I. du Pont de Nemours and Company. The purchase of this product conveys to the buyer the nontransferable right to use the purchased amount of the product for research and development only, including services for a third party for consideration. The buyer cannot sell or otherwise transfer this product, its components or materials made using this product or its components to a third party. Information about licenses for excluded uses is available from: E. I. du Pont de Nemours and Company; Attn: Associate Director, Commercial Development; DuPont Experimental Station E268; 200 Powdermill Rd.; Wilmington, DE 19803; 1-877-881-9787 (voice), 1-302-695-1437 (fax), licensing@dupont.com.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Distinguishing between Leucine and Isoleucine by Integrated LC-MS Analysis Using an Orbitrap Fusion Mass Spectrometer.
Xiao Y, et al.
Analytical Chemistry, 88, 10757-10766 (2016)
Vital Vialas et al.
Journal of proteomics, 152, 138-149 (2016-12-19)
Despite the maturity reached by targeted proteomic strategies, reliable and standardized protocols are urgently needed to enhance reproducibility among different laboratories and analytical platforms, facilitating a more widespread use in biomedical research. To achieve this goal, the use of dimensionless
A multicentric study to evaluate the use of relative retention times in targeted proteomics.
Vialas V, et al.
Journal of Proteomics, 152, 138-149 (2017)
Yongsheng Xiao et al.
Analytical chemistry, 88(21), 10757-10766 (2016-11-02)
Despite the great success of mass spectrometry (MS) for de novo protein sequencing, Leu and Ile have been generally considered to be indistinguishable by MS because their molecular masses are exactly the same. Positioning of incorrect Leu/Ile residues in variable
Mohd Moin Khan et al.
Current research in immunology, 1, 10-22 (2021-04-06)
Cancerous inhibitor of protein phosphatase 2A (CIP2A) is involved in immune response, cancer progression, and Alzheimer's disease. However, an understanding of the mechanistic basis of its function in this wide spectrum of physiological and pathological processes is limited due to

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