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L2011

Sigma-Aldrich

D-Lactic Dehydrogenase from Lactobacillus leichmannii

ammonium sulfate suspension, ≥250 units/mg protein (biuret)

Synonym(s):

Lactate, (R)-Lactate:NAD+ oxidoreductase, D−LDH

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

form

ammonium sulfate suspension

Quality Level

specific activity

≥250 units/mg protein (biuret)

foreign activity

Malic dehydrogenase <0.5% of base activity

storage temp.

2-8°C

General description

Research area: Cell Signalling D-lactate dehydrogenase belongs to the D-isomer-specific 2-hydroxyacid dehydrogenase family. Lactate dehydrogenase (LD) is a tetramer consisting of two (H and M) subunits. It has five isozymes (LD1 to LD5) and this composition varies in different tissues. LD1 is abundant in the kidney, heart, and erythrocytes. LD5 is found in the liver and skeletal muscles. LD is a ubiquitous molecule found in plants, yeast, mammals, and microorganisms and is a member of the oxidoreductase family.

Biochem/physiol Actions

Lactic dehydrogenase (LDH)plays an important role in the glycolytic pathway where it converts pyruvate tolactate by using NAD+ as a co-factor. It is considered animportant molecule that can be used in cancer therapy as it acts as aglycolytic inhibitor. Inhibition of LDH is associated with blocking of aerobicglycolysis in tumour cells.

Unit Definition

One unit will reduce 1.0 μmole of pyruvate to D-lactate per min at pH 7.0 at 25 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4, pH 6.0

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Torben Larsen
Analytical biochemistry, 539, 152-157 (2017-11-06)
D-lactic acid in the mammalian body is mainly of microbiological origin and is often located somewhere along the digestive tract. Surgical, extensive re-sectioning of the small bowel may be one of the risk factors for altered balance in the microbiological
D L Zhang et al.
Postgraduate medical journal, 79(928), 110-112 (2003-03-04)
A 12 year old boy presented with 11 episodes of weakness, ataxia, nausea, slurred speech, dehydration, and sometimes severe lethargy bordering on coma. A year previously the boy had small intestinal resections leaving 20 cm of small bowel remaining. D-lactic
H Taguchi et al.
The Journal of biological chemistry, 266(19), 12588-12594 (1991-07-05)
The gene encoding D-lactate dehydrogenase (D-lactate: NAD+ oxidoreductase, EC 1.1.1.28) of Lactobacillus plantarum has been sequenced, and expressed in Escherichia coli cells with an inducible expression plasmid, in which the 5'-noncoding region of the gene was replaced with the tac
Monica Gallo et al.
Frontiers in bioscience (Landmark edition), 20(8), 1234-1249 (2015-05-12)
Despite the intense scientific efforts made, there are still many tumors that are difficult to treat and the percentage of patient survival in the long-term is still too low. Thus, new approaches to the treatment of cancer are needed. Cancer
C L McLaughlin et al.
Journal of dairy science, 92(6), 2758-2766 (2009-05-19)
A challenge model was used to evaluate a new approach to controlling acute acidosis. Acute acidosis reduces performance in both dairy and beef cattle and most often occurs as a consequence of ingestion of large amounts of readily fermentable starch

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