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I3146

Sigma-Aldrich

ITS Liquid Media Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

Human ITS Liquid Media, Insulin-transferrin-sodium selenite

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About This Item

UNSPSC Code:
12352205
NACRES:
NA.75
Pricing and availability is not currently available.

sterility

sterile-filtered

Quality Level

500

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable
single cell analysis: suitable

impurities

endotoxin, tested

shipped in

ambient

storage temp.

2-8°C

Related Categories

Application

ITS Liquid Media Supplement (100×) has been used as a media supplement to culture:
  • α mouse liver 12 (AML-12) cells[1]
  • rat fetal testis explants[2]
  • human dental pulp stem cells (DPSC)[3]

Biochem/physiol Actions

  • Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.[4]
  • Transferrin: an iron-transport protein.
  • Selenium: an essential trace element normally provided by serum.[5]

Components

Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration.

Other Notes

ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000366647
0000366647
0000359154
0000346703
0000269673

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Leonardo Ricotti et al.
Biomedical materials (Bristol, England), 6(3), 031001-031001 (2011-05-14)
Polyelectrolyte layer-by-layer (LbL) nanofilms are interesting polymeric structures, built by alternating adsorption of positively and negatively charged polyelectrolytes. They consist of multilayer sheets with nanometric overall thickness, and they can be used as supports and surface coatings for in vitro
Christine M Reich et al.
Veterinary research communications, 36(2), 139-148 (2012-03-07)
In the dog, mesenchymal stem cells (MSCs) have been shown to reside in the bone marrow (bone marrow-derived mesenchymal stem cells: BM-MSCs) as well as in the adipose tissue (adipose tissue-derived stem cells: ADSCs). Potential application fields for these multipotent
Yi Yu et al.
Scientific reports, 7(1), 1729-1729 (2017-05-13)
Hepatitis B virus (HBV) infection causes acute hepatitis B (AHB), chronic hepatitis B (CHB), liver cirrhosis (LC), and eventually hepatocellular carcinoma (HCC). The presence of hepatitis B e antigen (HBeAg) in the serum generally indicates ongoing viral replication and disease
Zhibin Lin et al.
Cell death & disease, 11(1), 58-58 (2020-01-25)
Translationally controlled tumor protein (TCTP), which is a protein characterized by its potent proliferation promoting activity, has been well studied in the area of growth and tumorigenesis. However, the specific role of TCTP in liver regeneration (LR) and its underlying
Qiang Gao et al.
Cell death & disease, 9(6), 602-602 (2018-05-24)
Long non-coding RNAs (lncRNAs) are a new class of regulators of various human diseases. This study was designed to explore the potential role of lncRNAs in experimental hepatic damage. In vivo hepatic damage in mice and in vitro hepatocyte damage
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Questions

1–3 of 3 Questions  

  1. What should you use to dilute this to a 1X solution?

    1 answer

    1. This product can be dissolved in a given target cell culture medium, e.g., non-complex media (MEM, RPMI-1640), to a 1X solution.

      Helpful?

  2. What are the product numbers for the stand alone components in I3146?

    1 answer

    1. The product numbers for the stand-alone components in I3146 are as follows:
      Insulin – I2643 or I3536 (sterile)
      Apo-Transferrin – T2252
      Sodium Selenite – S5261
      EBBS – E3024

      Helpful?

  3. The ITS product #I3146 has been on back order for a while. Is there an alternative with similar components?

    1 answer

    1. Based on the Media Supplement Formulation, the closest alternative to product No. I3146 is the I2521 ITS+1 Liquid Media Supplement (100×), which also contains BSA and Linoleic acid.

      Helpful?

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