The stability of this product after being diluted in medium, has not been evaluated. However, various medium products that are supplemented with Hydrocortisone, are stable for up to 6 weeks when stored refrigerated. As an example, see product C-20011, Keratinocyte Growth Medium, at the link below:
https://www.sigmaaldrich.com/US/en/product/sigma/c20011
H6909
Hydrocortisone solution
50 μM, sterile-filtered, BioXtra, suitable for cell culture
Synonym(s):
17-Hydroxycorticosterone, Cortisol
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352209
PubChem Substance ID:
NACRES:
NA.77
biological source:
non-animal source
form:
liquid
Pricing and availability is not currently available.
Recommended Products
biological source
non-animal source
Quality Level
sterility
sterile-filtered
product line
BioXtra
form
liquid
concentration
50 μM
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
5.0- 7.0
shipped in
dry ice
storage temp.
−20°C
SMILES string
C[C@]12CCC(=O)C=C1CC[C@H]3[C@@H]4CC[C@](O)(C(=O)CO)[C@@]4(C)C[C@H](O)[C@H]23
InChI
1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1
InChI key
JYGXADMDTFJGBT-VWUMJDOOSA-N
Gene Information
human ... ABCB1(5243) , CYP3A4(1576) , NR3C1(2908) , SERPINA6(866)
mouse ... Abcb1a(18671) , Abcb1b(18669) , Ifng(15978) , Nos2(18126)
rat ... Ar(24208) , Nr3c1(24413)
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Application
Other Notes
Animal-component free
Physical form
frozen in working aliquots, avoid repeated freeze/thaw
related product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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How long is this product stable in cell culture medium (e.g. DMEM) at 4°C, or just 4°C in general?
1 answer-
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Is it suitable to further dilute small quantities of Product H6909, Hydrocortisone solution, in phosphate-buffered saline to prepare lower concentration aliquots?
1 answer-
It is not recommended to further dilute H6909, Hydrocortisone solution, for storage purposes as stability/integrity of more dilute forms cannot be guaranteed.
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What is the concentration of Product No. H6909, Hydrocortisone solution?
1 answer-
Product No. H6909 is a 50 μM solution. This may also be written as approximately 0.0181 g/L.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Product No. H6909, Hydrocortisone solution, isotonic?
1 answer-
Product No. H6909 is a solution of hydrocortisone in normal saline (0.85%). It is an isotonic solution of hydrocortisone.
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Should Product No. H6909, Hydrocortisone solution, be protected from light?
1 answer-
Yes. Product No. H6909 is packaged in an amber vial. If making single use aliquots, we recommend to use amber vials to protect the solution from light.
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Is there ethanol in Product No. H6909, Hydrocortisone solution?
1 answer-
Hydrocortisone is initially solubilized in ethanol and then diluted in normal saline. The final concentration of ethanol is 2 ml/L (0.2% v/v).
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