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CS0011

Sigma-Aldrich

Phospholipase C (PLC) Activity Assay Kit

Sufficient for 100 colorimetric tests

Synonym(s):

Phospholipase C assay

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

Quality Level

200

shipped in

dry ice

storage temp.

−20°C

General description

Phosphatidylcholine-specific phospholipase C (PC-PLC) is one of the important members of phospholipase family which is capable of specifically hydrolyzing the third phosphate linker of glycerophospholipid molecules, releasing phosphocholine and diacylglycerols (DAG).

PC-PLC has a wide range of biological functions and is involved in various cell signalling pathways, including apoptosis, proliferation, differentiation, motility, gene expression and metastasis. Furthermore, it has been demonstrated that PC-PLC plays a crucial role in the development of atherosclerosis, making PC-PLC an interesting target for the development of cardiovascular drugs. Therefore, it is desirable to identify novel inhibitors of PC-PLC and to analyze their influence on PC-PLC.
Phospholipase C (PLC) Activity Assay Kit provides a simple, rapid and sensitive procedure for measuring PLC activity in various sample types. The kit can detect as low as 0.03 mU/well of PLC activity. The kit can detect PC-PLC activity of purified enzymes and PC-PLC activity in cell extracts. The kit can be used in screening PC-PLC inhibitors.

p-Nitrophenylphosphorylcholine is a chromogenic substrate that is used in this kit to measure the activity of PLC. Hydrolysis of p-nitrophenylphosphorylcholine by PLC results in the liberation of p-nitrophenol, which can be measured at 405 nm at pH 7.2-7.5. The colorimetric signal is proportional to the PLC activity.
Supportive calculator (Click here to download a calculator excel file): Will calculate the reagents needed according to your experimental needs.

Storage Class Code

10 - Combustible liquids

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Certificates of Analysis (COA)

Lot/Batch Number
0000113868

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Questions

1–2 of 2 Questions  

  1. Can this kit be used to detect the phospholipase activity of certain microorganisms?

    1 answer

    1. This kit has only been evaluated with PC-PLC from mammalian sources.

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  2. Can this kit be used to measure PLC activity in human whole blood?

    1 answer

    1. This kit is tested using cell lysates. It has not been tested on whole blood, however the dark color of whole blood would very likely mask results. It may be suitable for use with serum or plasma. The minimum limit of detection is 0.03 mU. It would be up to the end user to determine suitability.

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