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S7107

Sigma-Aldrich

ApopTag TdT Enzyme

ApopTag Terminal deoxynucleotidyl Transferase (TdT) is a recombinant enzyme intended for use in labeling the 3′-OH ends of fragmented DNA during apoptotic cell detection.

Synonym(s):

TdT enzyme, Terminal deoxynucleotidyl transferase

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

Quality Level

manufacturer/tradename

ApopTag
Chemicon®

shipped in

dry ice

General description

ApopTag Terminal deoxynucleotidyl Transferase (TdT) is a recombinant enzyme intended for use in labeling the 3′-OH ends of fragmented DNA during apoptotic cell detection. Qualified for use with Apoptag In Situ Apoptosis Detection Kits (Catalog #s: S7100, S7101, S7110, S7111, S7160 and S7165).
ApopTag is a registered trademark of Serologicals Company.

Application

Materials Provided:
Each vial contains 300 μl of ApopTag Terminal Transferase (TdT) Enzyme.
Instructions for Use

Prepare labeling mixture as follows:
77 μl ApopTag Reaction Buffer (S7105 or kit component # 02)
33 μl ApopTag TdT Enzyme
Mix well and keep on ice. This reagent may be prepared in advance and stored on ice for no more than 6 hours. The Reaction Buffer - TdT Enzyme mixture is ready for use at the "APPLY WORKING STRENGTH TdT ENZYME" step in The Complete ApopTag Manual.
Warnings and Precautions :
ApopTag Terminal Transferase (TdT) Enzyme contains potassium cacodylate (dimethylarsinic acid) as a buffer. This material is harmful if swallowed; avoid contact with skin and eyes (wear gloves, glasses); wash areas of contact immediately.

Packaging

1000 units/mL

Storage and Stability

Recommended Storage -15°C to -25°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Health hazardEnvironment

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Carc. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3


Certificates of Analysis (COA)

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