MABE302
Anti-2,2,7-Trimethylguanosine Antibody, clone K121
clone K121, from mouse
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
K121, monoclonal
species reactivity
human, mouse
technique(s)
affinity chromatography: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
isotype
IgG1κ
shipped in
wet ice
target post-translational modification
unmodified
General description
The 2,2,7-trimethylguanosine cap structure is found on the 5’ end of small nuclear RNAs and small nucleolar RNAs. Initially, these RNA molecules are capped with 7-monomethylguanosine (m7G) which is then methylated by the trimethylguanosine synthase enzyme to form 2,2,7-trimethylguanosine. The synthesis of 2,2,7-trimethylguanosine occurs in the cytoplasm and is an important marker for nuclear localization of RNA molecules. Capping of RNAs is an important requirement for RNA transport and splicing processes. Uncapped RNAs are rapidly degraded by the 5′ exoribonuclease enzyme. 2,2,7-trimethylguanosine capping may play a role in the expression of viral RNAs.
Application
Anti-2, 2, 7-Trimethylguanosine Antibody, clone K121 is a high quality Mouse Monoclonal Antibody for the detection of 2, 2, 7-Trimethylguanosine & has been validated in ICC, PAI & IP.
Immunoaffinity Purification Analysis: A representative lot from an independent laboratory was used in IAP (Krainer, A.R., et al. (1988). Nucleic Acids Res. 16(20):9415-9429.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory was immunoprecipitated in IP (Moketi, S., et al. (2002). Mol Cell. 10(3):599-609.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory was immunoprecipitated in IP (Moketi, S., et al. (2002). Mol Cell. 10(3):599-609.).
Quality
Evaluated by Immunocytochemistry in NIH/3T3, HeLa, and A431 cells.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected 2,2,7-trimethylguanosine in NIH/3T3, HeLa, and A431 cells.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected 2,2,7-trimethylguanosine in NIH/3T3, HeLa, and A431 cells.
Physical form
Format: Purified
Analysis Note
Control
NIH/3T3, HeLa, and A431 cells
NIH/3T3, HeLa, and A431 cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Cyrille Girard et al.
Nucleic acids research, 34(10), 2925-2932 (2006-06-02)
Neuronal degeneration in spinal muscular atrophy (SMA) is caused by reduced expression of the survival of motor neuron (SMN) protein. The SMN protein is ubiquitously expressed and is present both in the cytoplasm and in the nucleus where it localizes
Magdalena Strzelecka et al.
Nucleus (Austin, Tex.), 1(1), 96-108 (2011-02-18)
The Cajal body (CB) is an evolutionarily conserved nuclear subcompartment, enriched in components of the RNA processing machinery. The composition and dynamics of CBs in cells of living organisms is not well understood. Here we establish the zebrafish embryo as
Cristina Moreno-Castro et al.
Journal of cell science, 132(22) (2019-10-23)
Cajal bodies are nuclear organelles involved in the nuclear phase of small nuclear ribonucleoprotein (snRNP) biogenesis. In this study, we identified the splicing factor TCERG1 as a coilin-associated factor that is essential for Cajal body integrity. Knockdown of TCERG1 disrupts
Ranjodh Sandhu et al.
Cell research, 23(4), 537-551 (2013-03-13)
Telomerase is a ribonucleoprotein enzyme typically required for sustained cell proliferation. Although both telomerase activity and the telomerase catalytic protein component, TbTERT, have been identified in the eukaryotic pathogen Trypanosoma brucei, the RNA molecule that dictates telomere synthesis remains unknown.
Jennifer Porat et al.
Nature communications, 13(1), 1277-1277 (2022-03-13)
The telomerase holoenzyme is critical for maintaining eukaryotic genome integrity. In addition to a reverse transcriptase and an RNA template, telomerase contains additional proteins that protect the telomerase RNA and promote holoenzyme assembly. Here we report that the methyl phosphate
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