MAB3067
Anti-Connexin 43 Antibody, clone 4E6.2
clone 4E6.2, Chemicon®, from mouse
Synonym(s):
Gap junction 43 kDa heart protein, connexin 43, gap junction protein, alpha 1, 43kDa, gap junction protein, alpha 1, 43kDa (connexin 43), gap junction protein, alpha-like
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4E6.2, monoclonal
species reactivity
mouse, rat, pig, canine
species reactivity (predicted by homology)
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... GJA1(2697)
General description
Connexins are highly homologous proteins that assemble as hexamers (connexons) to form gap junctions, small channels which allow for the passage of ions and other small molecules up to 1,200 daltons between neighboring cells. Gap junctions play an important role in a variety of cellular processes including homeostasis, intercellular metabolic cooperation and synchronization, morphogenesis, cell differentiation, and growth control. The most broadly studied connexins are the 26, 32, 43 and 45 kDa isoforms. Connexin 43 is a 382 a.a. protein belonging to the alpha-type (Group II) subfamily of connexin proteins and possessing four transmembrane regions, with cytoplasmic amino and carboxyl terminals. It undergoes rapid turnover in the cell and its monomers may reside in the ER/Golgi network, forming a reservoir available for assembly upon degradation of existing connexin-43 channels. Connexin 43 has been detected in a variety of tissues including: atrial and ventricular myocytes, granulose cells, astrocytes, fibroblasts, leptomeningeal cells, brain, lung, smooth muscle, uterine muscle, kidney, lens, epithelium, cornea and testis. Connexin 43 also may have a role in communication between migrating cardiac neural crest cells.
Specificity
Connexin-43. Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse brain lysate. Mouse connexin 43 sequence is identical in rat and bovine, and differs by one amino acid in pig. This sequence has no known homology with other connexins. Also effective for detection of Cx43 in mouse keratinocyte cells and on frozen sections of rat heart.
Immunogen
A synthetic peptide corresponding to positions 252-270 of the mouse connexin 43 sequence. This sequence is identical in rat and bovine.
Epitope: C-terminus
Application
Anti-Connexin 43 Antibody, clone 4E6.2 detects level of Connexin 43 & has been published & validated for use in ELISA, IC, IH & WB.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
Western Blot (colorimetric detection):
1:1000-10,000 freshly prepared lysates, proteinase inhibitors are recommended. Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse brain lysate.
Immunocytochemistry:
A 1:50-1:250 dilution of a previous lot was used in immunocytochemistry.
Immunohistochemistry:
A previous lot was used to detect Connexin 43 expression in the keratinocytes of the middle and upper epidermal layers of frozen human skin tissue (Arita, Ken, et al. (2006). Am. J. Pathol. 169:416-423).
Immunohistochemistry:
A previous lot was used to detect Connexin 43 expression in human and rat heart tissue (1:500 dilution).
ELISA:
A previous lot of this antibody was used in ELISA.
Optimal working dilutions must be determined by end user.
1:1000-10,000 freshly prepared lysates, proteinase inhibitors are recommended. Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse brain lysate.
Immunocytochemistry:
A 1:50-1:250 dilution of a previous lot was used in immunocytochemistry.
Immunohistochemistry:
A previous lot was used to detect Connexin 43 expression in the keratinocytes of the middle and upper epidermal layers of frozen human skin tissue (Arita, Ken, et al. (2006). Am. J. Pathol. 169:416-423).
Immunohistochemistry:
A previous lot was used to detect Connexin 43 expression in human and rat heart tissue (1:500 dilution).
ELISA:
A previous lot of this antibody was used in ELISA.
Optimal working dilutions must be determined by end user.
Quality
Evaluated by Western Blot on rat brain lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected Connexin 43 on 10 μg of rat brain lysates
Western Blot Analysis:
1:1000 dilution of this antibody detected Connexin 43 on 10 μg of rat brain lysates
Target description
43-47 kDa
Physical form
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 liquid in PBS containing sodium azide.
Storage and Stability
Stable for 1 year at 2-8ºC in undiluted aliquots from date of receipt.
Analysis Note
Control
Mouse brain tissue lysate
Mouse brain tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Rengasayee Veeraraghavan et al.
Pflugers Archiv : European journal of physiology, 467(10), 2093-2105 (2015-01-13)
It has long been held that electrical excitation spreads from cell-to-cell in the heart via low resistance gap junctions (GJ). However, it has also been proposed that myocytes could interact by non-GJ-mediated "ephaptic" mechanisms, facilitating propagation of action potentials in
Farida Goubaeva et al.
Biochemical and biophysical research communications, 352(1), 97-103 (2006-11-17)
Connexin 43 (Cx43) is thought to be present largely in the plasma membrane and its function solely to provide low resistance electrical connection between myocytes. A recent report suggested the presence of Cx43 in the mitochondria as well. We confirmed
Susi Zatti et al.
Molecular therapy. Methods & clinical development, 1, 1-1 (2014-01-01)
Duchenne muscular dystrophy (DMD)-associated cardiac diseases are emerging as a major cause of morbidity and mortality in DMD patients, and many therapies for treatment of skeletal muscle failed to improve cardiac function. The reprogramming of patients' somatic cells into pluripotent
Rengasayee Veeraraghavan et al.
Pflugers Archiv : European journal of physiology, 468(10), 1651-1661 (2016-08-12)
It was recently demonstrated that cardiac sodium channels (Nav1.5) localized at the perinexus, an intercalated disc (ID) nanodomain associated with gap junctions (GJ), may contribute to electrical coupling between cardiac myocytes via an ephaptic mechanism. Impairment of ephaptic coupling by
Katharina Grikscheit et al.
Cell communication & adhesion, 15(1), 185-193 (2008-07-24)
In the human heart, ventricular myocytes express connexin 43 (Cx43) and traces of Cx45. In congestive heart failure, Cx43 levels decrease, Cx45 levels increase and gap junction size decreases. To determine whether alterations of connexin coexpression ratio influence gap junction
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