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Key Documents

1.25072

Millipore

Fractogel® EMD SO3 ⁻ (M) Resin

MiniChrom Column Fractogel® SO3 (M), volume 5 mL, prepacked column

Synonym(s):

MiniChrom Column Fractogel® SO3 (M), 5 ml

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About This Item

UNSPSC Code:
41115711

ligand

(Sulfoisobutyl)

Quality Level

material

polypropylene housing

product line

Fractogel®

form

prepacked column

parameter

≤8 bar pressure
~200 cm/hr max. flow rate

column I.D. × L

8 mm × 100 mm

volume

5 mL

matrix active group

Methacrylate

mean particle size

40-90 μm

capacity

~130 mL, resin binding capacity

application(s)

mAb
recombinant protein
vaccine development

separation technique

cation exchange

storage temp.

15-25°C

General description

8 x 100mm

Features and Benefits

MiniChrom Column Fractogel® SO3 (M), 5 ml enables:
  • Increased binding strength at low pH
  • High aggregate removal efficiency combined with high dynamic binding capacity
  • Lower elution volumes for the highest purity levels
  • Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution


Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel® EMD SO3- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:
  • the buffer system
  • pH value of the buffer which determines the surface charge of the protein
  • the concentration of the counter ions
  • the charge density on the support (protein binding capacity)

Packaging

1 pkg Plastic bag(s)

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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