1.11732

Trypan blue (C.I. 23850)

for microscopy

• Synonym(s): Trypan Blue, Benzamine blue, Tolidine-disazo-bis-8-amino-1-naphthol-3,6-disulfonic acid tetrasodium salt, 3,3′-Dimethyl-4,4′-bis(5-amino-4-hydroxy-2,7-disulfonaphthyl-3-azo)-[1,1′-biphenyl] tetrasodium salt, Direct blue 14
• Linear Formula: C₃₄H₂₄N₆O₁₄S₄Na₄
• CAS Number: 72-57-1
• Molecular Weight: 960.81
• Colour Index Number: 23850
• Beilstein: 4360496
• MDL number: MFCD00003969
• UNSPSC Code: 41116130
• EC Index Number: 200-786-7

Properties

• Quality Level: 400
• form: solid
• potency: 6200 mg/kg LD₅₀, oral (Rat)
• composition: Dye, ≥80% spectrophotometric assay
• pH: 9.8 (20 °C, 10 g/L in H₂O)
• mp: >300 °C (lit.)
• solubility: 10 g/L
• bulk density: 800 kg/m³
• εmax: 650-820 at 590-600 nm in water at 0.01 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• SMILES string: [Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
• InChI: 1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
• InChI key: GLNADSQYFUSGOU-UHFFFAOYSA-J

Description

General description

Trypan blue (C.I. 23850) for microscopy, is a dry dye for the preparation of a color staining solution, staining histological sample material of human origin, e.g. trichrome staining in collagenous connective tissue.
The dye is a CE certified IVD product and has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining.

Analysis Note

Dye content (spectrophotometrically): ≥ 80 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 590 - 600 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.01 g/l; water): 650 - 820
TLC-Test: passes test
Loss on drying (110 °C): ≤ 15 %
Suitability for microscopy: passes test

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H350
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 1B
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3